LOCUS       Exported                4714 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFS102-SV40-Galpha15, complete sequence.
ACCESSION   MF688633
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human 
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and 
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, 
            Switzerland
REFERENCE   3  (bases 1 to 4714)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Cloning vector pFS102-SV40-Galpha15"
                     /mol_type="other DNA"
                     /db_xref="taxon:2138155"
     CDS             15..1139
                     /codon_start=1
                     /product="G alpha 16"
                     /label=G alpha 16
                     /protein_id="AVR59180.1"
                     /translation="MARSLTWRCCPWCLTEDEKAAARVDQEINRILLEQKKQDRGELKL
                     LLLGPGESGKSTFIKQMRIIHGAGYSEEERKGFRPLVYQNIFVSMRAMIEAMERLQIPF
                     SRPESKHHASLVMSQDPYKVTTFEKRYAAAMQWLWRDAGIRAYYERRREFHLLDSAVYY
                     LSHLERITEEGYVPTAQDVLRSRMPTTGINEYCFSVQKTNLRIVDVGGQKSERKKWIHC
                     FENVIALIYLASLSEYDQCLEENNQENRMKESLALFGTILELPWFKSTSVILFLNKTDI
                     LEEKIPTSHLATYFPSFQGPKQDAEAAKRFILDMYTRMYTGCVDGPEGSKKGARSRRLF
                     SHYTCATDTQNIRKVFKDVRDSVLARYLDEINLL"
     regulatory      1177..1368
                     /regulatory_class="polyA_signal_sequence"
                     /note="simian virus 40 polyA"
     polyA_signal    1186..1307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1972..2560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2731..3591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3592..3696)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3723..4178
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    4309..4357
                     /note="synthetic polyadenylation signal"
     misc_feature    4371..4462
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     regulatory      4506..4714
                     /regulatory_class="promoter"
                     /note="simian virus 40 early promoter; PSV40"
     promoter        4517..4713
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4564..4699
                     /label=SV40 ori
                     /note="SV40 origin of replication"