LOCUS       Exported                3084 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFS-sigHis-PGK, complete sequence.
ACCESSION   JF313343
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Wilke S, Gossen M, Wirth D, Buessow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2011) Structural Biology, Helmholtz Centre for 
            Infection Research, Inhoffenstr. 7, Braunschweig 38124, Germany
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Cloning vector pFS-sigHis-PGK"
                     /mol_type="other DNA"
                     /db_xref="taxon:1043696"
     misc_recomb     25..72
                     /note="F3 site; synthetic variant 3 of the yeast 
                     flp-recombinase target (FRT) site"
     regulatory      85..94
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      91..147
                     /regulatory_class="other"
                     /note="signal peptide from Mus musculus IgG heavy chain"
     misc_feature    148..175
                     /label=BpiI cloning site
                     /note="BpiI cloning site"
     misc_feature    176..196
                     /label=C-terminal 6xHis tag
                     /note="C-terminal 6xHis tag"
     CDS             176..193
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    211..345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(253..270)
                     /label=pEFGM
                     /note="pEFGM"
     regulatory      675..1194
                     /regulatory_class="promoter"
                     /note="derived from Homo sapiens phosphoglycerate kinase 1 
                     gene"
     promoter        683..1193
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     misc_recomb     1220..1267
                     /label=wild type flp-recombinase target (FRT) site
                     /note="wild type flp-recombinase target (FRT) site"
     protein_bind    1220..1267
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      1273..1946
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     rep_origin      complement(1332..1920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2091..2951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase precursor"
                     /label=bla
                     /protein_id="AEI54556.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2091..2951)
                     /gene="bla"
                     /label=bla
     promoter        complement(2952..3056)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3067..3084
                     /label=pBR1
                     /note="pBR1"