LOCUS       Exported                8006 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pFRT, complete sequence.
ACCESSION   AY268481
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Gortchakov AA, Schwartz YB, Demakov SA.
  TITLE     A new convenient Drosophila transformation vector bearing tandem 
            functional FRT sites - pFRT
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Gortchakov AA, Schwartz YB, Demakov SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2003) Lab. Molecular Cytogenetics, The Institute 
            of Cytology and Genetics, SD RAS, Lavrentjeva 10, Novosibirsk 
            630090, Russia
REFERENCE   3  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Transformation vector pFRT"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:227483"
     misc_feature    1..587
                     /label=P element 5 end
                     /note="P element 5 end"
     misc_feature    1..586
                     /label=P element 5 end
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /allele="+mC"
                     /product="eye pigment precursor transporter"
                     /label=white
                     /protein_id="AAO92306.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     gene            complement(1364..4246)
                     /gene="white"
                     /allele="+mC"
                     /label=white
     misc_binding    4776..4842
                     /label=Flp recombinase binding site
                     /bound_moiety="Flp recombinase"
                     /note="FRT; Flp recombinase target"
     protein_bind    4785..4832
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    4843..4892
                     /note="MCS; multiple cloning site"
     misc_binding    4893..4959
                     /label=Flp recombinase binding site
                     /bound_moiety="Flp recombinase"
                     /note="FRT; Flp recombinase target"
     protein_bind    4902..4949
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    5014..5246
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    5247..5748
                     /label=white linker sequence
                     /note="white linker sequence"
     misc_feature    5749..7747
                     /label=derived from pUC8
                     /note="derived from pUC8"
     promoter        6048..6152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6153..7013
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7184..7772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7779..8006
                     /label=white linker sequence
                     /note="white linker sequence"