LOCUS       Exported                9848 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFosill-4, complete sequence.
ACCESSION   JX069764
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina
  JOURNAL   Genome Res. 22 (11), 2241-2249 (2012)
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   Williams LJS., Tabbaa DG, Li N, Berlin AM, Shea TP, MacCallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and 
            Harvard, 320 Charles Street, Cambridge, MA 02141, USA
REFERENCE   3  (bases 1 to 9848)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Cloning vector pFosill-4"
                     /mol_type="other DNA"
                     /db_xref="taxon:1206910"
     primer_bind     9..42
                     /note="SBS12 Illumina sequence; designed for amplification 
                     of Illumina fosmid library"
     promoter        complement(56..73)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      57..73
                     /regulatory_class="promoter"
                     /note="SP6 promoter; transcript counterclockwise"
     CDS             complement(213..872)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="camR; confers resistance to chloramphenicol"
                     /protein_id="AFN40099.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(213..872)
                     /gene="cat"
                     /label=cat
     promoter        complement(873..975)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1814..1880
                     /note="ori2; oriS"
     rep_origin      1899..2118
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2209..2964
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein E"
                     /label=repE
                     /protein_id="AFN40100.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            2209..2964
                     /gene="repE"
                     /label=repE
     misc_feature    2967..3217
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3543..4718
                     /codon_start=1
                     /gene="sopA"
                     /product="plasmid partition protein A"
                     /label=sopA
                     /note="stability of plasmid"
                     /protein_id="AFN40101.1"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3543..4718
                     /gene="sopA"
                     /label=sopA
     CDS             4718..5689
                     /codon_start=1
                     /gene="sopB"
                     /product="plasmid partition protein B"
                     /label=sopB
                     /note="stability of plasmid"
                     /protein_id="AFN40102.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            4718..5689
                     /gene="sopB"
                     /label=sopB
     misc_feature    5762..6235
                     /note="sopC; repeat region bound by SopB"
     misc_feature    6494..7164
                     /label=lambda cos site
                     /note="lambda cos site"
     misc_feature    6495..6894
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        7301..7405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7406..8266
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR; confers resistance to ampicillin"
                     /protein_id="AFN40098.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            7406..8266
                     /gene="bla"
                     /label=bla
     rep_origin      8437..9025
                     /note="pUC19 origin of replication; clockwise; RNAII -35 to
                     RNA/DNA switch"
     misc_feature    9296..9694
                     /label=lambda cos site
                     /note="lambda cos site"
     protein_bind    9712..9749
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP site"
     protein_bind    9712..9745
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        9761..9779
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9761..9778
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     misc_feature    9778
                     /note="T7 promoter transcript start; clockwise"
     primer_bind     9796..9828
                     /note="SBS3 Illumina sequence; designed for amplification 
                     of Illumina fosmid library"