LOCUS       Exported                9863 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFosill-3, complete sequence.
ACCESSION   JX069763
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina
  JOURNAL   Genome Res. 22 (11), 2241-2249 (2012)
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Williams LJS., Tabbaa DG, Li N, Berlin AM, Shea TP, MacCallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and 
            Harvard, 320 Charles Street, Cambridge, MA 02141, USA
REFERENCE   3  (bases 1 to 9863)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Cloning vector pFosill-3"
                     /mol_type="other DNA"
                     /db_xref="taxon:1206909"
     primer_bind     21..60
                     /note="SBS8 Illumina sequence; designed for amplification 
                     of Illumina fosmid library"
     promoter        complement(71..88)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      72..88
                     /regulatory_class="promoter"
                     /note="SP6 promoter; transcript counterclockwise"
     CDS             complement(228..887)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="camR; confers resistance to chloramphenicol"
                     /protein_id="AFN40094.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(228..887)
                     /gene="cat"
                     /label=cat
     promoter        complement(888..990)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1829..1895
                     /note="ori2; oriS"
     rep_origin      1914..2133
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2224..2979
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein E"
                     /label=repE
                     /protein_id="AFN40095.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            2224..2979
                     /gene="repE"
                     /label=repE
     misc_feature    2982..3232
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3558..4733
                     /codon_start=1
                     /gene="sopA"
                     /product="plasmid partition protein A"
                     /label=sopA
                     /note="stability of plasmid"
                     /protein_id="AFN40096.1"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3558..4733
                     /gene="sopA"
                     /label=sopA
     CDS             4733..5704
                     /codon_start=1
                     /product="plasmid partition protein B"
                     /label=plasmid partition protein B
                     /note="stability of plasmid"
                     /protein_id="AFN40097.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5777..6250
                     /note="sopC; repeat region bound by SopB"
     misc_feature    6509..7179
                     /label=lambda cos site
                     /note="lambda cos site"
     misc_feature    6510..6909
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        7316..7420
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7421..8281
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR; confers resistance to ampicillin"
                     /protein_id="AFN40093.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            7421..8281
                     /gene="bla"
                     /label=bla
     rep_origin      8452..9040
                     /note="pUC19 origin of replication; clockwise; RNAII -35 to
                     RNA/DNA switch"
     misc_feature    9311..9709
                     /label=lambda cos site
                     /note="lambda cos site"
     protein_bind    9727..9760
                     /label=Cre recombinase binding site
                     /bound_moiety="Cre recombinase"
                     /note="loxP site"
     promoter        9776..9794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9776..9793
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     misc_feature    9793
                     /note="T7 promoter transcript start; clockwise"
     primer_bind     9811..9843
                     /note="SBS3 Illumina sequence; designed for amplification 
                     of Illumina fosmid library"