LOCUS       Exported                3756 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFNK-503-qscrsaL, complete sequence.
ACCESSION   MH300674
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
  PUBMED    29891706
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Karig DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns 
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins 
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 3756)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Cloning vector pFNK-503-qscrsaL"
                     /mol_type="other DNA"
                     /db_xref="taxon:2231586"
     CDS             complement(67..786)
                     /codon_start=1
                     /product="LasR"
                     /label=LasR
                     /protein_id="AWV91930.1"
                     /translation="MALVDGFLELERSSGKLEWSAILQKMASDLGFSKILFGLLPKDSQ
                     DYENAFIVGNYPAAWREHYDRAGYARVDPTVSHCTQSVLPIFWEPSIYQTRKQHEFFEE
                     ASAAGLVYGLTMPLHGARGELGALSLSVEAENRAEANRFMESVLPTLWMLKDYALQSGA
                     GLAFEHPVSKPVVLTSREKEVLQWCAIGKTSWEISVICNCSEANVNFHMGNIRRKFGVT
                     SRRVAAIMAVNLGLITL"
     regulatory      complement(796..804)
                     /regulatory_class="ribosome_binding_site"
                     /note="RBS II"
     regulatory      complement(816..871)
                     /regulatory_class="promoter"
     regulatory      complement(825..830)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(848..853)
                     /regulatory_class="minus_35_signal"
     regulatory      907..963
                     /regulatory_class="promoter"
                     /note="prokaryotic; qscrsaL"
     misc_feature    907..926
                     /label=las box - qscrsaL
                     /note="las box - qscrsaL"
     RBS             1001..1012
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      1001..1009
                     /regulatory_class="ribosome_binding_site"
                     /note="RBSII"
     CDS             1019..1774
                     /codon_start=1
                     /product="GFP(LVA)"
                     /label=GFP
                     /protein_id="AWV91931.1"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRPAANDENYALVA"
     CDS             1019..1732
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      1819..1923
                     /regulatory_class="terminator"
                     /note="T1"
     terminator      1823..1909
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      2076..2638
                     /label=p15A ori
                     /note="p15A ori"
     rep_origin      complement(2078..2623)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      complement(2732..2837)
                     /regulatory_class="terminator"
                     /note="T0"
     terminator      2737..2831
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2862..3656)
                     /codon_start=1
                     /product="Kan(r)"
                     /label=Kan
                     /protein_id="AWV91932.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"