LOCUS       Exported                3866 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFNK-202-qsc119, complete sequence.
ACCESSION   MH300673
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
  PUBMED    29891706
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Karig DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns 
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins 
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 3866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Cloning vector pFNK-202-qsc119"
                     /mol_type="other DNA"
                     /db_xref="taxon:2231585"
     CDS             complement(135..860)
                     /codon_start=1
                     /product="RhlR"
                     /label=RhlR
                     /protein_id="AWV91927.1"
                     /translation="MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAY
                     GVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQS
                     RMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLT
                     QKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKK
                     FDAPNKTLAAAYAAALGLI"
     RBS             867..878
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      complement(870..878)
                     /regulatory_class="ribosome_binding_site"
                     /note="RBSII"
     regulatory      complement(884..939)
                     /regulatory_class="promoter"
     regulatory      complement(893..898)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(916..921)
                     /regulatory_class="minus_35_signal"
     misc_feature    1018..1037
                     /label=qsc119 rhl box
                     /note="qsc119 rhl box"
     RBS             1092..1103
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      1092..1100
                     /regulatory_class="ribosome_binding_site"
                     /note="RBSII"
     CDS             1110..1865
                     /codon_start=1
                     /product="GFP(LVA)"
                     /label=GFP
                     /protein_id="AWV91928.1"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRPAANDENYALVA"
     CDS             1110..1823
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      1928..2032
                     /regulatory_class="terminator"
                     /note="T1"
     terminator      1932..2018
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      2186..2748
                     /label=p15A ori
                     /note="p15A ori"
     rep_origin      complement(2188..2733)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     regulatory      complement(2842..2947)
                     /regulatory_class="terminator"
                     /note="T0"
     terminator      2847..2941
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2972..3766)
                     /codon_start=1
                     /product="Kan(r)"
                     /label=Kan
                     /protein_id="AWV91929.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQAIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"