LOCUS       Exported                3669 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFM46, complete sequence.
ACCESSION   JQ394801
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Anderson JC, Voigt CA, Arkin AP.
  TITLE     Environmental signal integration by a modular AND gate
  JOURNAL   Mol. Syst. Biol. 3, 133 (2007)
  PUBMED    17700541
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Salis HM, Mirsky EA, Voigt CA.
  TITLE     Automated design of synthetic ribosome binding sites to control 
            protein expression
  JOURNAL   Nat. Biotechnol. 27 (10), 946-950 (2009)
  PUBMED    19801975
REFERENCE   3  (bases 1 to 3669)
  AUTHORS   Tamsir A, Tabor JJ, Voigt CA.
  TITLE     Robust multicellular computing using genetically encoded NOR gates 
            and chemical wires
  JOURNAL   Nature 469 (7329), 212-215 (2011)
  PUBMED    21150903
REFERENCE   4  (bases 1 to 3669)
  AUTHORS   Moser F, Voigt CA.
  TITLE     Genetic Circuit Performance under Conditions Relevant for Industrial
            Bioreactors
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3669)
  AUTHORS   Anderson JC, Moser F, Tamsir A, Salis H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   6  (bases 1 to 3669)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Cloning vector pFM46"
                     /mol_type="other DNA"
                     /label=derived from Escherichia coli strain DH10b
                     /note="derived from Escherichia coli strain DH10b"
                     /db_xref="taxon:1175536"
     gene            150..974
                     /gene="p15A"
                     /label=p15A
     rep_origin      complement(413..957)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      1447..1490
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1493..1514
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     regulatory      1515..1549
                     /regulatory_class="promoter"
                     /note="BBa_J23102"
     gene            1558..2432
                     /gene="BBa_E0240"
                     /label=BBa_E0240
     regulatory      1558..1570
                     /regulatory_class="ribosome_binding_site"
                     /note="BBa_B0032"
     CDS             1577..2293
                     /codon_start=1
                     /gene="GFP"
                     /product="GFP"
                     /label=GFP
                     /note="BBa_E0040"
                     /protein_id="AFI26421.1"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            1577..2293
                     /gene="GFP"
                     /label=GFP
     regulatory      2305..2384
                     /regulatory_class="terminator"
                     /note="BBa_B0010"
     terminator      2313..2384
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      2393..2433
                     /regulatory_class="terminator"
                     /note="BBa_B0012"
     terminator      2400..2427
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    2434..2454
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(2672..3487)
                     /codon_start=1
                     /gene="KanR"
                     /product="KanR"
                     /label=KanR
                     /protein_id="AFI26422.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(2672..3487)
                     /gene="KanR"
                     /label=KanR