LOCUS       Exported                3742 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFLEV, complete sequence.
ACCESSION   KX156843
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Hernandez-Garcia CM, Bouchard RA, Rushton PJ, Jones ML, Chen X, 
            Timko MP, Finer JJ.
  TITLE     High level transgenic expression of soybean (Glycine max) GmERF and 
            Gmubi gene promoters isolated by a novel promoter analysis pipeline
  JOURNAL   BMC Plant Biol. 10, 237 (2010)
  PUBMED    21050446
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Finer JJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2016) Horticulture and Crop Science, The Ohio 
            State University, 1680 Madison Ave., Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 3742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Expression vector pFLEV"
                     /mol_type="other DNA"
                     /db_xref="taxon:1858796"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             462..1181
                     /codon_start=1
                     /gene="sGFP"
                     /product="GFP"
                     /label=sGFP
                     /protein_id="ANJ20930.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     gene            462..1181
                     /gene="sGFP"
                     /label=sGFP
     regulatory      1214..1489
                     /regulatory_class="terminator"
     terminator      1214..1466
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1521..1537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1545..1561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1569..1599)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1614..1635
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1923..2511)
                     /direction=LEFT
     CDS             complement(2682..3542)
                     /codon_start=1
                     /gene="AmpR"
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="ANJ20929.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2682..3542)
                     /gene="AmpR"
                     /label=AmpR
     promoter        complement(3543..3647)
                     /gene="bla"
                     /label=AmpR promoter