LOCUS       Exported                6116 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  FLAG-tagging vector pFLAG97-TSR, complete sequence.
ACCESSION   EU043160
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Human cytomegalovirus protein kinase UL97 forms a complex with the 
            tegument phosphoprotein pp65
  JOURNAL   J. Virol. 81 (19), 10659-10668 (2007)
  PUBMED    17634236
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2007) Biological Chemistry & Molecular 
            Pharmacology, Harvard Medical School, 250 Longwood Ave, Boston, MA 
            02115, USA
REFERENCE   3  (bases 1 to 6116)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="FLAG-tagging vector pFLAG97-TSR"
                     /mol_type="other DNA"
                     /label=two-step Red recombination plasmid
                     /note="two-step Red recombination plasmid"
                     /db_xref="taxon:459231"
     rep_origin      171..759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1017..1035
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1057..1375
                     /label=UL96 derived from human cytomegalovirus
                     /note="UL96 derived from human cytomegalovirus"
     CDS             join(1496..3125,4158..4675)
                     /codon_start=1
                     /product="FLAG-UL97"
                     /label=FLAG-UL97
                     /note="protein kinase; ganciclovir phosphotranferase; 
                     derived from human cytomegalovirus"
                     /protein_id="ABS86030.1"
                     /translation="MDYKDDDDKSSALRSRARSASLGTTTQGWDPPPLRRPSRARRRQW
                     MREAAQAAAQAAVQAAQAAAAQVAQAHVDENEVVDLMADEAGGGVTTLTTLSSVSTTTV
                     LGHATFSACVRSDVMRDGEKEDAASDKENLRRPVVPSTSSRGSAASGDGYHGLRCRETS
                     AMWSFEYDRDGDVTSVRRALFTGGSDPSDSVSGVRGGRKRPLRPPLVSLARTPLCRRRV
                     GGVDAVLEENDVELRAESQDSAVASGPGRIPQPLSGSSGEESATAVEADSTSHDDVHCT
                     CSNDQIITTSIRGLTCDPRMFLRLTHPELCELSISYLLVYVPKEDDFCHKICYAVDMSD
                     ESYRLGQGSFGEVWPLDRYRVVKVARKHSETVLTVWMSGLIRTRAAGEQQQPPSLVGTG
                     VHRGLLTATGCCLLHNVTVHRRFHTDMFHHDQWKLACIDSYRRAFCTLADAIKFLNHQC
                     RVCHFDITPMNVLIDVNPHNPSEIVRAALCDYSLSEPYPDYNERCVAVFQETGTARRIP
                     NCSHRLRECYHPAFRPMPLQKLLICDPHARFPVAGLRRYCMSELSALGNVLGFCLMRLL
                     DRRGLDEVRMGTEALLFKHAGAACRALENGKLTHCSDACLLILAAQMSYGACLLGEHGA
                     ALVSHTLRFVEAKMSSCRVRAFRRFYHECSQTMLHEYVRKNVERLLATSDGLYLYNAFR
                     RTTSIICEEDLDGDCRQLFPE"
     misc_feature    1499..1522
                     /label=Region: FLAG epitope octapeptide
                     /note="Region: FLAG epitope octapeptide"
     CDS             1499..1522
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    3081..3125
                     /note="45-bp repeat; Red recombination substrate"
     misc_feature    3126..3143
                     /label=I-SceI homing endonuclease recognition site
                     /note="I-SceI homing endonuclease recognition site"
     CDS             3267..4082
                     /codon_start=1
                     /product="AphAI"
                     /label=AphAI
                     /note="aminoglycoside phosphotransferase; provides 
                     kanamycin resistance; derived from Escherichia coli 
                     kanamycin-resistant Tn10-based transposon cassette in 
                     GenBank Accession Number AJ601386"
                     /protein_id="ABS86031.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    4113..4157
                     /note="45-bp repeat; Red recombination substrate"
     misc_feature    4769..4785
                     /note="UL96 derived from human cytomegalovirus; alkaline 
                     exonuclease"
     misc_feature    4786..1056
                     /note="pSP72 backbone; vector from Promega Inc."
     promoter        complement(4787..4805)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        5151..5255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5256..6116
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"