LOCUS       Exported                3005 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFL747, complete sequence.
ACCESSION   AF287730
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Caspers MP, Lok F, Sinjorgo KM, van Zeijl MJ, Nielsen KA, 
            Cameron-Mills V.
  TITLE     Synthesis, processing and export of cytoplasmic 
            endo-beta-1,4-xylanase from barley aleurone during germination
  JOURNAL   Plant J. 26 (2), 191-204 (2001)
  PUBMED    11389760
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Lok F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2000) Dept. of Physiology, Carlsberg Laboratory, 
            Gl. Carlsbergvej 10, Valby DK-2500, Denmark
REFERENCE   3  (bases 1 to 3005)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Expression vector pFL747"
                     /mol_type="other DNA"
                     /label=plasmid pXYN/[XYN1-213/GFP/XYN502-556]
                     /note="plasmid pXYN/[XYN1-213/GFP/XYN502-556]"
                     /db_xref="taxon:161559"
     CDS             join(1007..1024,1108..1629,1721..2728)
                     /codon_start=1
                     /product="endo-xylanase GFP fusion protein"
                     /label=endo-xylanase GFP fusion protein
                     /note="derived from Hordeum vulgare endo-xylanase gene"
                     /protein_id="AAK73566.1"
                     /translation="MASTTQDVNMDGNLAGCVPFGTGTTTLSVHIEEEMAMLPVTVAVG
                     GNKPSGRYVLVAGRADEEDGLRLPIPVDTLKPRLTYRVAGWISLGAARGTSHPVRIDLG
                     VEDNGNETLVECGAVCAKEGGWSEIMGAFRLRTEPRSAAVYVHGAPAGVDVKVMDLRVY
                     PVDHKARFRQLKDKTDKARKRDVILKLGTPAGAGAGAAASVRVVQLDNAFGSVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQ
                     NTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGTQKE
                     WKTDARGNFDGDGNFKFRGFYGRYVVEVTTAKGKQILKTFRVEKGDSTPLVVDLADA"
     mRNA            join(1007..1024,1108..1629,1721..2728)
                     /product="endo-xylanase GFP fusion protein"
                     /label=endo-xylanase GFP fusion protein mRNA
     CDS             1826..2539
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     terminator      2745..2997
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"