LOCUS       Exported                3524 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFL746, complete sequence.
ACCESSION   AF287729
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Caspers MP, Lok F, Sinjorgo KM, van Zeijl MJ, Nielsen KA, 
            Cameron-Mills V.
  TITLE     Synthesis, processing and export of cytoplasmic 
            endo-beta-1,4-xylanase from barley aleurone during germination
  JOURNAL   Plant J. 26 (2), 191-204 (2001)
  PUBMED    11389760
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Lok F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2000) Dept. of Physiology, Carlsberg Laboratory, 
            Gl. Carlsbergvej 10, Valby DK-2500, Denmark
REFERENCE   3  (bases 1 to 3524)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Expression vector pFL746"
                     /mol_type="other DNA"
                     /label=plasmid pAMY6-4[AMY1-29/GFP/AMY419-423]
                     /note="plasmid pAMY6-4[AMY1-29/GFP/AMY419-423]"
                     /db_xref="taxon:161558"
     CDS             join(1007..1093,2531..3247)
                     /codon_start=1
                     /product="alpha-amylase GFP fusion protein"
                     /label=alpha-amylase GFP fusion protein
                     /note="derived from Hordeum vulgare amylase gene"
                     /protein_id="AAK73565.1"
                     /translation="MANKHLSLSLFLVLLGLSASLASGQVLFQVSKGEELFTGVVPILV
                     ELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFTYGVQCFSRYP
                     DHMKQHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDG
                     NILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPV
                     LLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     mRNA            join(1007..1093,2531..3247)
                     /product="alpha-amylase GFP fusion protein"
                     /label=alpha-amylase GFP fusion protein mRNA
     CDS             2531..3244
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     terminator      3264..3516
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"