LOCUS       Exported                3027 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  Expression vector pFL728, complete sequence.
ACCESSION   AF287728
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Caspers MP, Lok F, Sinjorgo KM, van Zeijl MJ, Nielsen KA, 
            Cameron-Mills V.
  TITLE     Synthesis, processing and export of cytoplasmic 
            endo-beta-1,4-xylanase from barley aleurone during germination
  JOURNAL   Plant J. 26 (2), 191-204 (2001)
  PUBMED    11389760
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Lok F.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2000) Dept. of Physiology, Carlsberg Laboratory, 
            Gl. Carlsbergvej 10, Valby DK-2500, Denmark
REFERENCE   3  (bases 1 to 3027)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Expression vector pFL728"
                     /mol_type="other DNA"
                     /label=plasmid pAMY6-4/XYN [1-501]
                     /note="plasmid pAMY6-4/XYN [1-501]"
                     /db_xref="taxon:161557"
     regulatory      1..744
                     /regulatory_class="promoter"
                     /note="derived from Hordeum vulgare amylase gene"
     mRNA            join(745..762,846..1367,1459..2424)
                     /product="truncated 1,4-beta-D xylan xylanohydrolase"
                     /label=truncated 1,4-beta-D xylan xylanohydrolase mRNA
     CDS             join(745..762,846..1367,1459..2424)
                     /codon_start=1
                     /product="truncated 1,4-beta-D xylan xylanohydrolase"
                     /label=truncated 1,4-beta-D xylan xylanohydrolase
                     /note="derived from Hordeum vulgare; truncated C-55"
                     /protein_id="AAK73564.1"
                     /translation="MASTTQDVNMDGNLAGCVPFGTGTTTLSVHIEEEMAMLPVTVAVG
                     GNKPSGRYVLVAGRADEEDGLRLPIPVDTLKPRLTYRVAGWISLGAARGTSHPVRIDLG
                     VEDNGNETLVECGAVCAKEGGWSEIMGAFRLRTEPRSAAVYVHGAPAGVDVKVMDLRVY
                     PVDHKARFRQLKDKTDKARKRDVILKLGTPAGAGAGAAASVRVVQLDNAFPFGTCINTS
                     VIQKPAFLDFFTNHLDWAVFENELKWYHTEVQQGQLNYADADALLAFCDRLGKTVRGHC
                     VFWSVDGDVQQWVKNLNKDQLRSAMQSRLEGLVSRYAGRFKHYDVNNEMLHGRFFRDRL
                     GDEDVPAYMFKEVARLDPEPALFVNDYNVECGNDPNATPEKYAEQVAWLQSCGAVVRGI
                     GLQGHVQNPVGEVICAALDRLAKTGVPIWFTELDVPEYDVGLRAKDLEVVLREAYAHPA
                     VEGIVFWGFMQGTMWRQNAWLVDADGTVNEAGQMFLNLQKEWK"