LOCUS       Exported                6398 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  Food-grade vector pFGV356N DNA, complete sequence.
ACCESSION   AB119528
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Kushiro A, Kiwaki M, Shimizu K.
  TITLE     A cryptic plasmid of Lactobacillus casei and construction of shuttle
            vector and food-grade vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Kushiro A, Kiwaki M, Shimizu K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2003) Akira Kushiro, Yakult Central Institute for 
            Microbiological Research, Clinical Microbiology; 1796, Yaho, 
            Kunitachi, Tokyo 186-8650, Japan (E-mail:akira-kushiro@yakult.co.jp,
            Tel:81-42-577-8962(ex.2403), Fax:81-42-577-3020)
REFERENCE   3  (bases 1 to 6398)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Food-grade vector pFGV356N"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:244935"
     source          4112..6398
                     /organism="Lactobacillus casei"
                     /plasmid="pYIT356"
                     /mol_type="other DNA"
                     /db_xref="taxon:1582"
     gene            74..106
                     /gene="lacEGF"
                     /label=lacEGF
     regulatory      74..79
                     /regulatory_class="minus_35_signal"
                     /gene="lacEGF"
                     /inference="non-experimental evidence, no additional
                     details recorded"
     regulatory      101..106
                     /regulatory_class="minus_10_signal"
                     /gene="lacEGF"
                     /inference="non-experimental evidence, no additional
                     details recorded"
     gene            186..1919
                     /gene="lacE"
                     /label=lacE
     CDS             186..1919
                     /codon_start=1
                     /gene="lacE"
                     /product="enzyme II"
                     /label=lacE
                     /protein_id="BAD42834.1"
                     /translation="MNKVFDKLKPVFEAIAANKYISAIRDGFIACMPIIIFSSIFMMVA
                     YVPNAWGFYWPDNVTNTLMVAYKYSMGLLALFVAGTTAKNLTDSKNLELPKTNQINPVA
                     VIVASEISFVILSILPLKTGVDLTYMGTQGLICAYIVGLIVPNIYYVCIKNNVTIKLPA
                     QVPGNIAQSFKDLIPMGLSVTAFWLFGVGFKAATGTVLPRWIIQVLSPLFQASDSYLGL
                     ALIAGAMAFFWFCGVQGPSIVQPAVVPIMIANTAANLQQYQAGQHVSHVLAMNTMDYVM
                     NFGGTGSTFVVAFIMLFAARSAQLKAVVKAAFVPCTFGVNEPVLFGMPIIMNPMLFIPF
                     LATPIVNVCLFKFFVSVLGMNSMMYTMPWTVPGPIGILISTGFAPLAFVFVLLTLVLDV
                     AIYFPFIRVYDSTLLAEEKAKEEVIEDDGMAVLASDTVSPSIPTGLTVATATDDDATHV
                     LPETAPSAHGEAYFKQNEVDVLVLCAGGGTSGILANALNKLSKERGLKLSAAARAYGQD
                     MDLIKDMNMVILAPQMESMKGNLKKITDKYGVKLVTTTGRQYIELTNNGDMALDFVESN
                     L"
     gene            1946..3370
                     /gene="lacG"
                     /label=lacG
     CDS             1946..3370
                     /codon_start=1
                     /gene="lacG"
                     /product="phospho-beta-galactosidase"
                     /label=lacG
                     /protein_id="BAD42835.1"
                     /translation="MSKQLPQDFVMGGATAAYQVEGATKEDGKGRVLWDDFLDKQGRFK
                     PDPAADFYHRYDEDLALAEKYGHQVIRVSIAWSRIFPDGAGEVEPRGVAFYHKLFADCA
                     AHHIEPFVTLHHFDTPERLHEAGDWLSQEMLDDFVAYAKFCFEEFSEVKYWITINEPTS
                     MAVQQYTSGTFPPAESGRFDKTFQAEHNQMVAHARIVNLYKSMQLGGQIGIVHALQTVY
                     PYSDSAVDHHAAELQDALENRLYLDGTLAGEYHQETLALVKEILDANHQPMFQSTPQEM
                     KAIDEAAHQLDFVGVNNYFSKWLRAYHGKSETIHNGDGTKGSSVARLQGVGEEKLPDGI
                     ETTDWDWSIYPRGMYDILMRIHNDYPLVPVTYVTENGIGLKESLPENATPDTVIEDPKR
                     IDYVKKYLSAMADAIHDGANVKGYFIWSLQDQFSWTNGYSKRYGLFFVDFPTQNRYIKQ
                     SAEWFKSVSETHIIPD"
     gene            3419..3754
                     /gene="lacF"
                     /label=lacF
     CDS             3419..3754
                     /codon_start=1
                     /gene="lacF"
                     /product="enzyme III"
                     /label=lacF
                     /protein_id="BAD42836.1"
                     /translation="MATKEEISMVGFALVAYAGDARTAAVHALDAAEAGDFDKANELVE
                     KAQQDINEAHNQQTQLLSQEAGGAEMDVTFIMVHGQDTLMTTMLLIDETRYMIRMFKRI
                     KELENKQ"
     repeat_region   4757..4777
                     /label=DR1
                     /note="DR1"
     repeat_region   4766..4781
     repeat_region   4778..4798
                     /label=DR2
                     /note="DR2"
     repeat_region   4787..4802
     repeat_region   4799..4819
                     /label=DR3
                     /note="DR3"
     repeat_region   4808..4823
     repeat_region   4820..4840
                     /label=DR4
                     /note="DR4"
     repeat_region   4829..4844
     gene            4853..5737
                     /gene="repB"
                     /label=repB
     regulatory      4853..4858
                     /regulatory_class="minus_35_signal"
                     /gene="repB"
                     /inference="non-experimental evidence, no additional
                     details recorded"
     regulatory      4876..4881
                     /regulatory_class="minus_10_signal"
                     /gene="repB"
                     /inference="non-experimental evidence, no additional
                     details recorded"
     CDS             4925..5737
                     /codon_start=1
                     /gene="repB"
                     /product="Replication protein"
                     /label=repB
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="BAD42837.1"
                     /translation="MNVALAGLLDRQDYLVTQANDLARSLGNLSTFQHKVLDYCFSYVT
                     ANDNANTVYHLTTLEIIHHLGLNTSGDSYKRVVKAFKALNENTAIYMRTTEPDGKHGIL
                     MTSLFAYIKVIDDGRVEFKFSATVAPFVFQLKRQFYSFHLSELTRVRSKYTLSMMKLWN
                     ANAIGKWRDQNDPSSLPPAATIKGSLTDWESWFLGSDDDGKPIKWPAGRFKQKAIDVAI
                     KELGSLYPQTMINVLRLTHGRRIEGFQIEFRPIRTVLDLNPQVIDGKP"