LOCUS       Exported               11545 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pFGUS3, complete sequence.
ACCESSION   KC920579
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Regulation of NAC2 expression by miR164c mediates fertilizer 
            application-induced delay of leaf senescence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2013) Section of Molecular Cell & Developmental 
            Biology, The University of Texas at Austin, Austin, TX 78712, USA
REFERENCE   3  (bases 1 to 11545)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Suite 8
            Coverage              :: complete
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Binary vector pFGUS3"
                     /mol_type="other DNA"
                     /db_xref="taxon:1473577"
     misc_feature    1..27
                     /note="T-Border (Left); left border repeat from C58 T-DNA"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     3UTR           complement(111..362)
                     /note="MAS polyA; polyA signal from mannopine synthase"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="BAR"
                     /product="BAR"
                     /label=BAR
                     /note="Phosphinothricin acetyltransferase (PAT), conferring
                     tolerance to the herbicide phosphinothricin (PPT)"
                     /protein_id="AHN10447.1"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     gene            complement(373..924)
                     /gene="BAR"
                     /label=BAR
     regulatory      complement(930..1310)
                     /regulatory_class="promoter"
                     /note="MAS promoter; mannopine synthase promoter"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    1317..1578
                     /note="NOS terminator sequence to insulate MAS promoter 
                     from the promoter to be cloned"
     terminator      1325..1577
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1579..1681
                     /note="Multiple Cloning Sites (MCS); NOS terminator 
                     sequence to insulate MAS promoter from the promoter to be 
                     cloned"
     CDS             join(1818..2285,2476..4296)
                     /codon_start=1
                     /gene="HTB2-GUS"
                     /product="HTB2-GUS"
                     /label=HTB2-GUS
                     /protein_id="AHN10448.1"
                     /translation="MAKADKKPAEKKPAEKTPAAEPAAAAEKKPKAGKKLPKEPAGAGD
                     KKKKRSKKNVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAGESSKLARY
                     NKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSSAAAAAAMVDLRNRRLVRP
                     VETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAVPGSFNDQFADADIRNY
                     AGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVMEHQGGYTPFEADVTPYV
                     IAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFFNYAGIHRSVMLYTTPN
                     TWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVVATGQGTSGTLQVVNPH
                     LWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLINHKPFYFTGFGRHEDA
                     DLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWADEHGIVVIDETAAVGFN
                     LSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKNHPSVVMWSIANEPDTR
                     PQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSG
                     DLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHR
                     VFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFG
                     EKPQQGGKQ"
     gene            1818..4296
                     /gene="HTB2-GUS"
                     /label=HTB2-GUS
                     /note="Encoding fusion protein HTB2-GUS; a linker of 6 Ala 
                     added; GUS coding sequence from pFGUS2a"
     intron          2286..2475
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2491..4296
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="RPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIAV
                     PGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVME
                     HQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDFF
                     NYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQVV
                     ATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQFLI
                     NHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWAD
                     EHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDKN
                     HPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDLF
                     DVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYTD
                     MWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKPK
                     SAAFLLQKRWTGMNFGEKPQQGGKQ"
     misc_feature    4303..1310
                     /label=sequence from pFGCGW INSD accession DQ231581.1
                     /note="sequence from pFGCGW INSD accession DQ231581.1"
     3UTR           4303..5022
                     /note="OCS polyA; polyA signal from octopine synthase"
     terminator      4313..5020
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(5049..5065)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5268..5292
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    5268..5291
                     /note="T-Border (Right); right border repeat from C58 
                     T-DNA"
     rep_origin      complement(6353..7353)
                     /direction=LEFT
                     /label=pVS1 replication origin
                     /note="pVS1 replication origin"
     CDS             6592..7221
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7655..8722
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8788..8982
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9326..9466
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9652..10240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      9756..10037
                     /label=pBR325 replication origin
                     /note="pBR325 replication origin"
     CDS             complement(10327..11121)
                     /codon_start=1
                     /gene="aadA"
                     /product="kanamycin (R)"
                     /label=aadA
                     /note="aadA (kanamycin resistance) gene amplified from 
                     pIG121Hm"
                     /protein_id="AHN10449.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(10327..11121)
                     /gene="aadA"
                     /label=aadA