LOCUS       Exported                7338 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGLAHIL6T, complete sequence.
ACCESSION   LT727635
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7338)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Fungal expression vector pFGLAHIL6T"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 2236."
                     /db_xref="taxon:1944732"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      457..1565
                     /regulatory_class="promoter"
                     /note="A. niger glaA promoter"
     misc_feature    1566..1606
                     /label=5 UTR of A. niger glaA
                     /note="5 UTR of A. niger glaA"
     exon            1607..1820
                     /note="mutated exon 1 of A. niger glaA"
     intron          1821..1895
                     /note="intron 1 of A. niger glaA"
     exon            1896..2182
                     /note="exon 2 of A. niger glaA"
     intron          2183..2237
                     /note="intron 2 of A. niger glaA"
     exon            2238..2334
                     /note="exon 3 of A. niger glaA"
     intron          2335..2395
                     /note="intron 3 of A. niger glaA"
     exon            2396..3033
                     /note="exon 4 of A. niger glaA"
     intron          3034..3091
                     /note="intron 4 of A. niger glaA"
     exon            3092..3775
                     /note="exon 5 of A. niger glaA"
     CDS             3776..3790
                     /codon_start=1
                     /note="unnamed protein product; spMF"
                     /protein_id="SJL89047.1"
                     /translation="RMDKR"
     CDS             3791..4348
                     /codon_start=1
                     /note="unnamed protein product; hIL6"
                     /protein_id="SJL89048.1"
                     /translation="APVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETC
                     NKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQN
                     RFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMT
                     THLILRSFKEFLQSSLRALRQM"
     regulatory      4530..5099
                     /regulatory_class="terminator"
                     /note="A. nidulans trpC terminator"
     primer_bind     complement(5117..5133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5141..5157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(5160..5243)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(5165..5195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5210..5231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5335..5737)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(5519..6107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6278..7138)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL89049.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7139..7243)
                     /gene="bla"
                     /label=AmpR promoter