LOCUS       Exported                6080 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGA2, complete sequence.
ACCESSION   LT727585
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6080)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Fungal expression vector pFGA2"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 1728."
                     /db_xref="taxon:1944727"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      457..1565
                     /regulatory_class="promoter"
                     /note="A. niger glaA promoter"
     misc_feature    1566..1606
                     /label=5 UTR of A. niger glaA
                     /note="5 UTR of A. niger glaA"
     exon            1607..1820
                     /note="exon 1 of A. niger glaA"
     intron          1821..1895
                     /note="intron 1 of A. niger glaA"
     exon            1896..2182
                     /note="exon 2 of A. niger glaA"
     intron          2183..2237
                     /note="intron 2 of A. niger glaA"
     exon            2238..2334
                     /note="exon 3 of A. niger glaA"
     intron          2335..2395
                     /note="intron 3 of A. niger glaA"
     exon            2396..3033
                     /note="exon 4 of A. niger glaA"
     intron          3034..3091
                     /note="intron 4 of A. niger glaA"
     exon            3092..3778
                     /note="exon 5 of A. niger glaA"
     misc_feature    3779..3834
                     /label=3 UTR of A. niger glaA
                     /note="3 UTR of A. niger glaA"
     primer_bind     complement(3859..3875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3883..3899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(3902..3985)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(3907..3937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3952..3973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4077..4479)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(4261..4849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5020..5880)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88917.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5881..5985)
                     /gene="bla"
                     /label=AmpR promoter