LOCUS       Exported               10639 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGA1, complete sequence.
ACCESSION   LT727219
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10639)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10639)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Fungal expression vector pFGA1"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4480."
                     /db_xref="taxon:1944726"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    420..5015
                     /label=5 flanking region of A. niger glaA
                     /note="5 flanking region of A. niger glaA"
     regulatory      5016..6124
                     /regulatory_class="promoter"
                     /note="A. niger glaA promoter"
     misc_feature    6125..6165
                     /label=5 UTR of A. niger glaA
                     /note="5 UTR of A. niger glaA"
     exon            6166..6379
                     /note="exon 1 of A. niger glaA"
     intron          6380..6454
                     /note="intron 1 of A. niger glaA"
     exon            6455..6741
                     /note="exon 2 of A. niger glaA"
     intron          6742..6796
                     /note="intron 2 of A. niger glaA"
     exon            6797..6893
                     /note="exon 3 of A. niger glaA"
     intron          6894..6954
                     /note="intron 3 of A. niger glaA"
     exon            6955..7592
                     /note="exon 4 of A. niger glaA"
     intron          7593..7650
                     /note="intron 4 of A. niger glaA"
     exon            7651..8337
                     /note="exon 5 of A. niger glaA"
     misc_feature    8338..8393
                     /label=3 UTR of A. niger glaA
                     /note="3 UTR of A. niger glaA"
     primer_bind     complement(8418..8434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8442..8458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(8461..8544)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(8466..8496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8511..8532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8636..9038)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(8820..9408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9579..10439)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87795.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10440..10544)
                     /gene="bla"
                     /label=AmpR promoter