LOCUS       Exported                4092 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFF828, complete sequence.
ACCESSION   KM923749
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Farzadfard F, Lu TK.
  TITLE     Synthetic biology. Genomically encoded analog memory with precise in
            vivo DNA writing in living cell populations
  JOURNAL   Science 346 (6211), 1256272 (2014)
  PUBMED    25395541
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Farzadfard F, Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2014) Biological Engineering, MIT, 77 
            Massachusetts Avenue, NE47, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4092)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Cloning vector pFF828"
                     /mol_type="other DNA"
                     /db_xref="taxon:1572892"
     promoter        99..201
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             202..861
                     /codon_start=1
                     /product="CmR"
                     /label=CmR
                     /protein_id="AIZ09117.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      882..976
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      complement(990..1660)
                     /direction=LEFT
                     /label=colE1
                     /note="colE1"
     rep_origin      1064..1652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      complement(1803..1907)
                     /regulatory_class="terminator"
     terminator      1816..1902
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1932..2894)
                     /codon_start=1
                     /product="retron EC86 RNA-directed DNA polymerase-like 
                     protein"
                     /label=retron EC86 RNA-directed DNA polymerase-like pr
                     /protein_id="AIZ09115.1"
                     /translation="MKSAEYLNTFRLRNLGLPVMNNLHDMSKATRISVETLRLLIYTAD
                     FRYRIYTVEKKGPEKRMRTIYQPSRELKALQGWVLRNILDKLSSSPFSIGFEKHQSILN
                     NATPHIGANFILNIDLEDFFPSLTANKVFGVFHSLGYNRLISSVLTKICCYKNLLPQGA
                     PSSPKLANLICSKLDYRIQGYAGSRGLIYTRYADDLTLSAQSMKKVVKARDFLFSIIPS
                     EGLVINSKKTCISGPRSQRKVTGLVISQEKVGIGREKYKEIRAKIHHIFCGKSSEIEHV
                     RGWLSFILSVDSKSHRRLITYISKLEKKYGKNPLNKAKT"
     misc_feature    2914..3047
                     /note="template for Ec86 RT; msd(lacZ_ON)"
     misc_feature    complement(3037..3118)
                     /note="primer for the Ec86 RT; msr"
     regulatory      complement(3127..3179)
                     /regulatory_class="promoter"
     CDS             3327..4079
                     /codon_start=1
                     /product="LuxR"
                     /label=LuxR
                     /note="R-protein from Vibrio fischeri"
                     /protein_id="AIZ09116.1"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK
                     KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP
                     SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ
                     MKLNTTNRCQSISKAILTGAIDCPYFKN"