LOCUS Exported 5251 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Shuttle cosmid vector pFD666, complete sequence.
ACCESSION AF000429
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5251)
AUTHORS Auerswald EA, Ludwig G, Schaller H.
TITLE Structural analysis of Tn5
JOURNAL Cold Spring Harb. Symp. Quant. Biol. 45 (Pt 1), 107-113 (1981)
PUBMED 6271452
REFERENCE 2 (bases 1 to 5251)
AUTHORS Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H.
TITLE Nucleotide sequence and exact localization of the neomycin
phosphotransferase gene from transposon Tn5
JOURNAL Gene 19 (3), 327-336 (1982)
PUBMED 6295884
REFERENCE 3 (bases 1 to 5251)
AUTHORS Norrander J, Kempe T, Messing J.
TITLE Construction of improved M13 vectors using
oligodeoxynucleotide-directed mutagenesis
JOURNAL Gene 26 (1), 101-106 (1983)
PUBMED 6323249
REFERENCE 4 (bases 1 to 5251)
AUTHORS Poustka A, Rackwitz HR, Frischauf AM, Hohn B, Lehrach H.
TITLE Selective isolation of cosmid clones by homologous recombination in
Escherichia coli
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81 (13), 4129-4133 (1984)
PUBMED 6330743
REFERENCE 5 (bases 1 to 5251)
AUTHORS Yanisch-Perron C, Vieira J, Messing J.
TITLE Improved M13 phage cloning vectors and host strains: nucleotide
sequences of the M13mp18 and pUC19 vectors
JOURNAL Gene 33 (1), 103-119 (1985)
PUBMED 2985470
REFERENCE 6 (bases 1 to 5251)
AUTHORS Gibson TJ, Rosenthal A, Waterston RH.
TITLE Lorist6, a cosmid vector with BamHI, NotI, ScaI and HindIII cloning
sites and altered neomycin phosphotransferase gene expression
JOURNAL Gene 53 (2-3), 283-286 (1987)
PUBMED 3038694
REFERENCE 7 (bases 1 to 5251)
AUTHORS Gibson TJ, Coulson AR, Sulston JE, Little PF.
TITLE Lorist2, a cosmid with transcriptional terminators insulating vector
genes from interference by promoters within the insert: effect on
DNA yield and cloned insert frequency
JOURNAL Gene 53 (2-3), 275-281 (1987)
PUBMED 3301536
REFERENCE 8 (bases 1 to 5251)
AUTHORS Denis F, Brzezinski R.
TITLE An improved aminoglycoside resistance gene cassette for use in
gram-negative bacteria and Streptomyces
JOURNAL FEMS Microbiol. Lett. 65 (3), 261-264 (1991)
PUBMED 1655560
REFERENCE 9 (bases 1 to 5251)
AUTHORS Denis F, Brzezinski R.
TITLE A versatile shuttle cosmid vector for use in Escherichia coli and
actinomycetes
JOURNAL Gene 111 (1), 115-118 (1992)
PUBMED 1547947
REFERENCE 10 (bases 1 to 5251)
AUTHORS Servin-Gonzalez L, Sampieri AI, Cabello J, Galvan L, Juarez V,
Castro C.
TITLE Sequence and functional analysis of the Streptomyces
phaeochromogenes plasmid pJV1 reveals a modular organization of
Streptomyces plasmids that replicate by rolling circle
JOURNAL Microbiology 141 (Pt 10), 2499-2510 (1995)
PUBMED 7582009
REFERENCE 11 (bases 1 to 5251)
AUTHORS Denis F, Brzezinski R.
TITLE Direct Submission
JOURNAL Submitted (20-APR-1997) Immunology, IRCM, 110 Pine Avenue West,
Montreal, QC H2W 1R7, Canada
REFERENCE 12 (bases 1 to 5251)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5251
/organism="Shuttle cosmid vector pFD666"
/lab_host="Escherichia coli; Actinomycetes"
/mol_type="other DNA"
/db_xref="taxon:60245"
misc_feature 1..76
/label=polylinker
/note="polylinker"
regulatory complement(81..109)
/regulatory_class="promoter"
/note="T7 RNA polymerase promoter"
promoter complement(85..103)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 128..151
/regulatory_class="terminator"
/note="trpA transcription terminator"
CDS complement(185..979)
/codon_start=1
/gene="AphI"
/product="aminoglycoside phosphotransferase"
/label=AphI
/protein_id="AAB60865.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
gene complement(185..979)
/gene="AphI"
/label=AphI
regulatory complement(1022..1069)
/regulatory_class="promoter"
/note="synthetic promoter"
rep_origin complement(1081..1923)
/direction=LEFT
/label=ColE1 replication origin
/note="ColE1 replication origin"
rep_origin complement(1261..1849)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 1936..2157
/label=bacteriophage lambda COS site
/note="bacteriophage lambda COS site"
rep_origin 2165..2603
/label=M13 replication origin
/note="M13 replication origin"
rep_origin 2165..2593
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 2604..3251
/label=pJV1 replication origin
/note="pJV1 replication origin"
CDS 3252..4838
/codon_start=1
/gene="pJV1 rep"
/product="pJV1 rep replication factor"
/label=pJV1 rep
/protein_id="AAB60866.1"
/translation="MLNRVSGIDACGGCGRRVLDPDTGVIYAKSSRGYVVTIGLVRCGR
IWFCPECSSAIRRGRTEEIKTGALRHLAAGGTLAVVVLTARHNQTTDLDSLVAALWGGP
LLDDKGAPVLDRSGKPRRAPGAYQRMLTAPAFYGRPEARRTRKDGTQYVRPAEDGIRHR
IGYIGMVRAAEVTRSKKNGYHPHLNLLVFLGGELSGTPAKGDVVGHFEPSETDLGDWED
WLREMWAGALKRADPKFEPSTDCDTPGCKCKGKGHGVMVSIVRSADDVALIEYLTKNQD
GKRERPDSVDQDLEAAGAAAMETARLDSKTGRGRKSMTPFQILYRLWDIEVAGLDPDMA
EGYGTPKQLRAWWAQYEEALAGRRAIEWTRGLRRHVDLDGDDDEETDLQYVYEPEAAPL
DGGVVLTSDAMRLVVGADAELDLDDVVRAEAYYSAVDVVTGLGGRADHVRVATAEELAE
VQEVLFARTQERAEESRRQRRIAEHEAEQAAAHRKRQELARCLGLLVRQRGGTQDDSAA
DNFVAHIHANR"
gene 3252..4838
/gene="pJV1 rep"
/label=pJV1 rep
terminator 4950..4993
/label=bacterial terminator
/note="putative bacterial transcription terminator"
regulatory complement(4955..4994)
/regulatory_class="terminator"
/note="rrnC transcription terminator"
regulatory 5177..5224
/regulatory_class="promoter"
/note="SP6 RNA polymerase promoter"
promoter 5216..5234
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
