LOCUS       Exported                9400 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Bacteroides fragilis shuttle vector pFD1146, complete sequence.
ACCESSION   JQ776640
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Parker AC, Jeffrey Smith C.
  TITLE     Development of an IPTG inducible expression vector adapted for 
            Bacteroides fragilis
  JOURNAL   Plasmid 68 (2), 86-92 (2012)
  PUBMED    22487080
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Smith CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2012) Dept. Microbiology & Immunology, East 
            Carolina University, 600 Moye Blvd., Greenville, NC 27834, USA
REFERENCE   3  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Bacteroides fragilis shuttle vector pFD1146"
                     /mol_type="other DNA"
                     /db_xref="taxon:1175996"
     CDS             complement(786..1466)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label=repA
                     /note="replication protein for replication in Bacteroides"
                     /protein_id="AFI41181.1"
                     /translation="MILNRKIIFPLILFLWKIIVYFVENNISQKHIKMENKKAVKLTDF
                     QKKQENPFMKTSYRGIENHVVKKYKSNSGGDKRAVVALADTETGEVFKTSFIRQIEVDE
                     EQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMKPKNDLIIFNRKKCLEYTKYKTDKAVY
                     KGLAELVKAEIIARGPADNLWFINPLIVFNGDRVTFAKTYVSEKDFSCPKERRSRETTI
                     ITWL"
     gene            complement(786..1466)
                     /gene="repA"
                     /label=repA
     CDS             2552..3352
                     /codon_start=1
                     /gene="ermF"
                     /product="ErmF"
                     /label=ermF
                     /note="erythromycin resistance protein"
                     /protein_id="AFI41182.1"
                     /translation="MTKKKLPVRFTGQHFTIDKVLIKDAIRQANISNQDTVLDIGAGKG
                     FLTVHLLKIANNVVAIENDTALVEHLRKLFSDARNVQVVGCDFRNFAVPKFPFKVVSNI
                     PYGITSDIFKILMFESLGNFLGGSIVLQLEPTQKLFSRKLYNPYTVFYHTFFDLKLVYE
                     VGPESFLPPPTVKSALLNIKRKHLFFDFKFKAKYLAFISYLLEKPDLSVKTALKSIFRK
                     SQVRSISEKFGLNLNAQIVCLSPSQWLNCFLEMLEVVPEKFHPS"
     gene            2552..3352
                     /gene="ermF"
                     /label=ermF
     CDS             4208..4975
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /note="spectinomycin resistance protein"
                     /protein_id="AFI41183.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            4208..4975
                     /gene="aad9"
                     /label=aad9
     rep_origin      5818..6406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            6727..6836
                     /note="incP origin of transfer"
     CDS             6869..7240
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     protein_bind    7472..7493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7508..7538
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7546..7562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7570..7586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7611..7631
                     /label=LacI, LacO3 binding site
                     /bound_moiety="LacI, LacO3"
     regulatory      7678..7699
                     /regulatory_class="promoter"
                     /gene="cfxA"
     gene            7678..7699
                     /gene="cfxA"
                     /label=cfxA
     protein_bind    7712..7732
                     /label=LacI, LacO1 binding site
                     /bound_moiety="LacI, LacO1"
     protein_bind    7714..7730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8292..9374
                     /codon_start=1
                     /gene="lacI"
                     /product="LacI"
                     /label=lacI
                     /note="LacI repressor; derived from Escherichia coli"
                     /protein_id="AFI41184.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     gene            8292..9374
                     /gene="lacI"
                     /label=lacI