LOCUS       Exported                5039 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFC8K, complete sequence.
ACCESSION   DQ137255
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     pFC8K (HaloTag) CMV Flexi Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2005) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2005) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   4  (bases 1 to 5039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Oct 6, 2005 this sequence version replaced DQ137255.1.
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Cloning vector pFC8K"
                     /mol_type="other DNA"
                     /db_xref="taxon:341552"
     regulatory      1..742
                     /regulatory_class="promoter"
                     /note="CMV enhancer/promoter region"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          857..989
                     /note="chimeric intron"
     regulatory      1033..1052
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1087..1422
                     /codon_start=1
                     /product="barnase"
                     /label=barnase
                     /protein_id="AAZ81416.1"
                     /translation="MAQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLAD
                     VAPGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHY
                     QTFTKIR"
     CDS             1456..1467
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    1501..2379
                     /label=HaloTag fusion peptide tag
                     /note="HaloTag fusion peptide tag"
     regulatory      2504..2725
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     polyA_signal    2513..2634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3033..3827
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /protein_id="AAZ81417.1"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      3996..4032
                     /label=ColE1-derived origin of replication
                     /note="ColE1-derived origin of replication"
     rep_origin      complement(3999..4587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4703..4988
                     /label=cer site
                     /note="cer site"
     misc_feature    4703..4986
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"