LOCUS       Exported                6990 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression/transfer vector pFB-ZB, complete sequence.
ACCESSION   JF682518
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Savitsky P, Burgess-Brown N, Gileadi O.
  TITLE     Baculovirus expression/transfer vector pFB-ZB
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Savitsky P, Burgess-Brown N, Gileadi O.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2011) Structural Genomics Consortium, University 
            of Oxford, Old Road Campus Research Building, Roosevelt Drive, 
            Oxford, Oxfordshire OX3 7DQ, UK
REFERENCE   3  (bases 1 to 6990)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Baculovirus expression/transfer vector pFB-ZB"
                     /mol_type="other DNA"
                     /db_xref="taxon:997459"
     regulatory      1..129
                     /regulatory_class="promoter"
                     /note="polyhedrin"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    148..390
                     /label=TEV-cleavable N-terminal tag
                     /note="TEV-cleavable N-terminal tag"
     misc_feature    154..186
                     /label=decahistidine tag
                     /note="decahistidine tag"
     CDS             154..183
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    193..393
                     /label=Z tag
                     /note="Z tag"
     CDS             193..363
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     misc_feature    370..390
                     /label=TEV protease cleavage site (cut after Q)
                     /note="TEV protease cleavage site (cut after Q)"
     CDS             370..390
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    420..453
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        468..913
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             914..2335
                     /codon_start=1
                     /gene="SacB"
                     /product="levansucrase"
                     /function="converts sucrose to a toxic product; allows 
                     selection of recombinant plasmids on plates containing 5% 
                     sucrose"
                     /label=SacB
                     /protein_id="ADZ56698.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYKVPEFDSSTIKNISSAKGLDVWDSWPLQNTDGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     gene            914..2335
                     /gene="SacB"
                     /label=SacB
     polyA_signal    2577..2711
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2740..2905
                     /label=Tn7L
                     /note="Tn7L"
     mobile_element  2740..2905
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3089..3544
                     /label=f1
                     /note="f1"
     promoter        3571..3675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3676..4536
                     /codon_start=1
                     /gene="ampR"
                     /product="beta lactamase"
                     /label=ampR
                     /note="ampicillin resistance protein"
                     /protein_id="ADZ56697.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            3676..4536
                     /gene="ampR"
                     /label=ampR
     rep_origin      4681..5354
                     /label=pUC
                     /note="pUC"
     rep_origin      4707..5295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5598..5822
                     /label=Tn7R
                     /note="Tn7R"
     mobile_element  5598..5822
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5889..6422)
                     /codon_start=1
                     /gene="Gm(R)"
                     /product="gentamycin resistance protein"
                     /label=Gm(R)
                     /protein_id="ADZ56699.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            complement(5889..6422)
                     /gene="Gm(R)"
                     /label=Gm(R)
     promoter        complement(6611..6639)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"