LOCUS       Exported                9609 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFB-Neo-LacZ, complete sequence.
ACCESSION   AF396261
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Jaskula J, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2001) Technical Services, Stratagene, 11011 N 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9609
                     /organism="Cloning vector pFB-Neo-LacZ"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="control vector; part of the Viraport retroviral 
                     vector system"
                     /db_xref="taxon:167883"
     LTR             289..758
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    823..1180
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1245..1661
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1671..2044
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             5634..5663
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5768..5785
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     LTR             6642..7235
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    7446..7586
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7772..8360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8531..9391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9392..9496)
                     /gene="bla"
                     /label=AmpR promoter