LOCUS       Exported                4939 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pFastBac1-HM, complete sequence.
ACCESSION   AY598466
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Katagiri Y, Ingham KC.
  TITLE     Enhanced production of green fluorescent fusion proteins in a 
            baculovirus expression system by addition of secretion signal
  JOURNAL   BioTechniques 33 (1), 24-26 (2002)
  PUBMED    12139250
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Sarafanov A, Saenko E.
  TITLE     High-throughput optimization of protein expression in the 
            baculovirus system based on determination of relative expression 
            efficiency of viral stocks
  JOURNAL   Anal. Biochem. 328 (1), 98-100 (2004)
  PUBMED    15081917
REFERENCE   3  (bases 1 to 4939)
  AUTHORS   Sarafanov AG, Makogonenko EM, Pechik IV, Radtke KP, Khrenov AV, 
            Ananyeva NM, Strickland DK, Saenko EL.
  TITLE     Identification of coagulation factor VIII A2 domain residues forming
            the binding epitope for low-density lipoprotein receptor-related 
            protein
  JOURNAL   Biochemistry 45 (6), 1829-1840 (2006)
  PUBMED    16460029
REFERENCE   4  (bases 1 to 4939)
  AUTHORS   Sarafanov AG, Makogonenko EM, Andersen OM, Mikhailenko IA, Ananyeva 
            NM, Khrenov AV, Shima M, Strickland DK, Saenko EL.
  TITLE     Localization of the low-density lipoprotein receptor-related protein
            regions involved in binding to the A2 domain of coagulation factor 
            VIII
  JOURNAL   Thromb. Haemost. 98 (6), 1170-1181 (2007)
  PUBMED    18064310
REFERENCE   5  (bases 1 to 4939)
  AUTHORS   Sarafanov AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2004) Biochemistry, American Red Cross, Holland 
            Laboratory, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
REFERENCE   6  (bases 1 to 4939)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Baculovirus expression vector pFastBac1-HM"
                     /mol_type="other DNA"
                     /db_xref="taxon:274590"
     rep_origin      2..457
                     /label=F1
                     /note="F1"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             589..1449
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="AAT09788.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1594..2267
                     /label=region derived from pUC
                     /note="region derived from pUC"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2511..2735
                     /label=Tn7R
                     /note="Tn7R"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /product="gentamicin resistance protein"
                     /label=gentamicin resistance protein
                     /protein_id="AAT09789.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     regulatory      3904..4032
                     /regulatory_class="promoter"
                     /note="polyhedrin promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     RBS             4063..4074
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4081..4143
                     /codon_start=1
                     /product="signal sequence from honeybee melittin"
                     /label=melittin signal sequence
                     /translation="MKFLVNVALVFMVVYISYIYA"
     misc_feature    4081..4137
                     /label=milettin secretion signal
                     /note="milettin secretion signal"
     misc_feature    4156..4173
                     /label=6xHis-tag
                     /note="6xHis-tag"
     CDS             4156..4173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      4204..4213
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    4208..4239
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             4246..4275
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4429..4563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  4592..4757
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"