LOCUS       Exported                4980 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  BiFC vector pFAGN173, complete sequence.
ACCESSION   KC344841
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Gong Z, Lu H.
  TITLE     A new combination of fluorescent protein fragments suitable for 
            green-colored bimolecular fluorescence complementation (BiFC) assays
            in Schizosaccharomyces pombe
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Gong Z, Lu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2012) School of Life Science, Fudan University, 
            Room 618, Science Building, Handan Road 220, Shanghai 200433, China
REFERENCE   3  (bases 1 to 4980)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="BiFC vector pFAGN173"
                     /mol_type="other DNA"
                     /db_xref="taxon:1306194"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    303..317
                     /label=linker
                     /note="linker"
     misc_feature    318..830
                     /note="GN173 (encoding amino acid residues 2-172 of mEGFP)"
     misc_feature    831..857
                     /label=HA tag
                     /note="HA tag"
     CDS             831..857
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      867..1359
                     /regulatory_class="terminator"
                     /note="Saccharomyces cerevisiae LEU2 terminator"
     regulatory      1370..1748
                     /regulatory_class="promoter"
                     /note="Ashbya gossypii TEF1 promoter"
     gene            1405..2761
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1405..1748
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1749..2558
                     /codon_start=1
                     /product="kan"
                     /label=kan
                     /note="kanamycin resistance gene"
                     /protein_id="AGH62564.1"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      2564..2761
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     regulatory      2565..2796
                     /regulatory_class="terminator"
                     /note="Ashbya gossypii TEF1 terminator"
     promoter        complement(2869..2887)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3130..3749
                     /label=pBR322 origin
                     /note="pBR322 origin"
     rep_origin      complement(3145..3733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3904..4764)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /note="beta-lactamase"
                     /protein_id="AGH62565.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4765..4869)
                     /gene="bla"
                     /label=AmpR promoter