LOCUS       Exported                4182 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-VN-TRP1, complete sequence.
ACCESSION   EF210804
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Sung MK, Huh WK.
  TITLE     Bimolecular fluorescence complementation analysis system for in vivo
            detection of protein-protein interaction in Saccharomyces cerevisiae
  JOURNAL   Yeast 24 (9), 767-775 (2007)
  PUBMED    17534848
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Sung M-K., Huh W-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2007) School of Biological Sciences, Seoul 
            National University, Seoul 151-747, Republic of Korea
REFERENCE   3  (bases 1 to 4182)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Yeast tagging vector pFA6a-VN-TRP1"
                     /mol_type="other DNA"
                     /db_xref="taxon:422193"
     regulatory      72..81
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             78..596
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     terminator      620..807
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        843..990
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             991..1665
                     /codon_start=1
                     /gene="trp1"
                     /product="phosphoribosylanthranilate isomerase"
                     /label=trp1
                     /protein_id="ABN05238.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     gene            991..1665
                     /gene="trp1"
                     /label=trp1
     RBS             1739..1747
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(1820..1838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2096..2684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2855..3715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABN05239.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2855..3715)
                     /gene="bla"
                     /label=bla
     promoter        complement(3716..3820)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4166..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"