LOCUS       Exported                5104 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast tagging vector pFA6a-URA3-kanMX6, complete sequence.
ACCESSION   HQ154040
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Iwase M, Nishihama R, Vallen EA, Luo J, Fang X, Tong Z, Guttman J, 
            Meisel R, Hanna J, Bi E.
  TITLE     A URA3-Kan cassette: diverse gene modifications in Saccharomyces 
            cerevisiae without leaving a trace
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Nishihama R.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2010) Department of Genetics, Stanford University 
            School of Medicine, 300 Pasteur Drive, Stanford, CA 94305-5120, USA
REFERENCE   3  (bases 1 to 5104)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Yeast tagging vector pFA6a-URA3-kanMX6"
                     /mol_type="other DNA"
                     /db_xref="taxon:938153"
     gene            70..1235
                     /gene="URA3"
                     /label=URA3
     promoter        118..333
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             334..1137
                     /codon_start=1
                     /gene="URA3"
                     /product="Ura3p"
                     /label=URA3
                     /protein_id="ADU15672.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            1236..2675
                     /gene="kanMX6"
                     /label=kanMX6
     regulatory      1236..1624
                     /regulatory_class="promoter"
                     /gene="kanMX6"
     gene            1281..2637
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1281..1624
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1625..2434
                     /codon_start=1
                     /gene="kanMX6"
                     /product="kanamycin and geneticin resistance protein"
                     /label=kanMX6
                     /note="derived from Escherichia coli transposon Tn903"
                     /protein_id="ADU15673.1"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     regulatory      2435..2675
                     /regulatory_class="terminator"
                     /gene="kanMX6"
     terminator      2440..2637
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2742..2760)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3018..3606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3777..4637)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ADU15674.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4638..4742)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5088..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"