LOCUS       Exported                4477 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA6a-TRP1-PGAL1-HBH, complete sequence.
ACCESSION   DQ407931
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P.
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae
  JOURNAL   Yeast 23 (8), 623-632 (2006)
  PUBMED    16823883
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2006) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   3  (bases 1 to 4477)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2009) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   4  (bases 1 to 4477)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Aug 21, 2009 this sequence version replaced DQ407931.1.
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Cloning vector pFA6a-TRP1-PGAL1-HBH"
                     /mol_type="other DNA"
                     /label=pSP72 derivative
                     /note="pSP72 derivative"
                     /db_xref="taxon:374898"
     promoter        complement(1..19)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        365..469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             470..1330
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABD66501.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            470..1330
                     /gene="bla"
                     /label=bla
     rep_origin      1501..2089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2347..2365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2438..2446
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3 end of the 16S rRNA (Chen et al., 1994)"
     gene            2471..3342
                     /gene="TRP1"
                     /label=TRP1
     CDS             complement(2520..3194)
                     /codon_start=1
                     /product="TRP1"
                     /label=TRP1
                     /protein_id="ABD66502.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(3195..3342)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     regulatory      3348..3816
                     /regulatory_class="promoter"
                     /allele="pGAL1"
     promoter        3437..3878
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3437..3554
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     misc_feature    3908..4196
                     /label=HBH-tag
                     /note="HBH-tag"
     CDS             3925..3942
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4171..4188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4215..4402
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"