LOCUS       Exported                5170 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Tagging vector pFA6a-natMX6-P81nmt1-3FLAG, complete sequence.
ACCESSION   EU689031
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E.
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe
  JOURNAL   Biotechnol J 3 (9-10), 1280-1285 (2008)
  PUBMED    18729046
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Noguchi C, Garabedian M, Malik M, Noguchi E.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2008) Biochemistry and Molecular Biology, Drexel 
            University College of Medicine, 245 N 15th Street, MS 497, 
            Philadelphia, PA 19102, USA
REFERENCE   3  (bases 1 to 5170)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Tagging vector pFA6a-natMX6-P81nmt1-3FLAG"
                     /mol_type="other DNA"
                     /db_xref="taxon:554665"
     promoter        complement(1..19)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        365..469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             470..1330
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ACH53393.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            470..1330
                     /gene="bla"
                     /label=bla
     rep_origin      1501..2089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2347..2365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(2470..3592)
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     terminator      2470..2667
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(2673..3248)
                     /codon_start=1
                     /gene="nat"
                     /product="nourseothricin resistance"
                     /label=nat
                     /protein_id="ACH53394.1"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGERQALYMSMPCP"
     gene            complement(2673..3248)
                     /gene="nat"
                     /label=nat
     promoter        complement(3249..3592)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     regulatory      3633..4805
                     /regulatory_class="promoter"
                     /note="nmt1 promoter P81"
     promoter        3637..4795
                     /label=nmt1 P81 promoter
                     /note="mutant nmt1 promoter from Schizosaccharomyces pombe,
                     conferring weak thiamine-repressible expression"
     CDS             4810..4887
                     /codon_start=1
                     /product="3xFLAG"
                     /label=3xFLAG
                     /protein_id="ACH53395.1"
                     /translation="MDYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             4813..4884
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     terminator      4908..5095
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"