LOCUS       Exported                5000 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA6a-kanMX6-PGAL1-HBH, complete sequence.
ACCESSION   DQ407930
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P.
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae
  JOURNAL   Yeast 23 (8), 623-632 (2006)
  PUBMED    16823883
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2006) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   3  (bases 1 to 5000)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2009) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   4  (bases 1 to 5000)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Aug 21, 2009 this sequence version replaced DQ407930.1.
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Cloning vector pFA6a-kanMX6-PGAL1-HBH"
                     /mol_type="other DNA"
                     /label=pSP72 derivative
                     /note="pSP72 derivative"
                     /db_xref="taxon:374897"
     promoter        complement(1..19)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        365..469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             470..1330
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABD66499.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            470..1330
                     /gene="bla"
                     /label=bla
     rep_origin      1501..2089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2347..2365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(2470..3826)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      2470..2667
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(2673..3482)
                     /codon_start=1
                     /gene="kanamycin"
                     /product="kanamycin and geneticin (G418) resistance 
                     protein"
                     /label=kanamycin
                     /protein_id="ABD66500.1"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(2673..3482)
                     /gene="kanamycin"
                     /label=kanamycin
     promoter        complement(3483..3826)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     regulatory      3871..4339
                     /regulatory_class="promoter"
                     /allele="pGAL1"
     promoter        3960..4401
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3960..4077
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     misc_feature    4431..4719
                     /label=HBH-tag
                     /note="HBH-tag"
     CDS             4448..4465
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4694..4711
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4738..4925
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"