LOCUS       Exported                4742 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA6a-HTB-hphMX4, complete sequence.
ACCESSION   DQ407922
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Tagwerker C, Zhang H, Wang X, Larsen LS, Lathrop RH, Hatfield GW, 
            Auer B, Huang L, Kaiser P.
  TITLE     HB tag modules for PCR-based gene tagging and tandem affinity 
            purification in Saccharomyces cerevisiae
  JOURNAL   Yeast 23 (8), 623-632 (2006)
  PUBMED    16823883
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2006) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   3  (bases 1 to 4742)
  AUTHORS   Kaiser P, Tagwerker C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2006) Biological Chemistry, UC Irvine, 19182 
            Jamboree Blvd RM H136 Plumwood Bldg, Irvine, CA 92697, USA
REFERENCE   4  (bases 1 to 4742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Jul 20, 2006 this sequence version replaced DQ407922.3.
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Cloning vector pFA6a-HTB-hphMX4"
                     /mol_type="other DNA"
                     /label=pSP72 derivative
                     /note="pSP72 derivative"
                     /db_xref="taxon:374889"
     misc_feature    58..410
                     /label=HTB-tag
                     /note="HTB-tag"
     CDS             72..89
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             117..137
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      412..648
                     /regulatory_class="terminator"
                     /note="ADH1"
     terminator      431..618
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            693..2268
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        693..1036
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1037..2065
                     /codon_start=1
                     /product="hygromycin resistance protein"
                     /label=hygromycin resistance protein
                     /protein_id="ABD66483.2"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      2071..2268
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2380..2398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2656..3244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3415..4275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABD66484.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3415..4275)
                     /gene="bla"
                     /label=bla
     promoter        complement(4276..4380)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4726..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"