LOCUS       Exported                3877 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Tagging vector pFA6a-5FLAG-bleMX6, complete sequence.
ACCESSION   EU689028
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Noguchi C, Garabedian MV, Malik M, Noguchi E.
  TITLE     A vector system for genomic FLAG epitope-tagging in 
            Schizosaccharomyces pombe
  JOURNAL   Biotechnol J 3 (9-10), 1280-1285 (2008)
  PUBMED    18729046
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Noguchi C, Garabedian M, Malik M, Noguchi E.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAY-2008) Biochemistry and Molecular Biology, Drexel 
            University College of Medicine, 245 N 15th Street, MS 497, 
            Philadelphia, PA 19102, USA
REFERENCE   3  (bases 1 to 3877)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Tagging vector pFA6a-5FLAG-bleMX6"
                     /mol_type="other DNA"
                     /db_xref="taxon:554653"
     CDS             66..248
                     /codon_start=1
                     /product="5xFLAG"
                     /label=5xFLAG
                     /protein_id="ACH53384.1"
                     /translation="MDYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK
                     HMGAPLLNKRISYDL"
     CDS             69..200
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     regulatory      215..443
                     /regulatory_class="terminator"
                     /note="ADH1"
     terminator      227..414
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            489..1410
                     /label=bleMX6
                     /note="yeast selectable marker conferring phleomycin 
                     resistance"
     promoter        489..832
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             833..1207
                     /codon_start=1
                     /gene="ble"
                     /product="bleomycin resistance protein"
                     /label=ble
                     /protein_id="ACH53385.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     gene            833..1207
                     /gene="ble"
                     /label=ble
     terminator      1213..1410
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1515..1533)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1791..2379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2550..3410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ACH53386.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2550..3410)
                     /gene="bla"
                     /label=bla
     promoter        complement(3411..3515)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3861..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"