LOCUS       Exported                5412 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFA-pHluorin-ARG4, complete sequence.
ACCESSION   JN051501
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Actin dynamics and endocytosis in the absence of Arp2/3 in Candida 
            albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur 
            Penfield, Montreal, Quebec H3A 1B1, Canada
REFERENCE   3  (bases 1 to 5412)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Cloning vector pFA-pHluorin-ARG4"
                     /mol_type="other DNA"
                     /label=derived from Candida albicans
                     /note="derived from Candida albicans"
                     /db_xref="taxon:1043212"
     gene            37..768
                     /gene="pHluorin"
                     /label=pHluorin
                     /note="pH-sensitive GFP variant for use in C. albicans; 
                     C-terminal fusion protein separated by triple Gly-Ala 
                     linker"
     regulatory      771..1024
                     /regulatory_class="terminator"
                     /note="Saccharomyces cerevisiae URA3 terminator"
     CDS             1482..2888
                     /codon_start=1
                     /gene="ARG4"
                     /product="Arg4"
                     /label=ARG4
                     /protein_id="AEI30145.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     gene            1482..2888
                     /gene="ARG4"
                     /label=ARG4
                     /note="selectable marker ARG4 from C. albicans"
     promoter        complement(3050..3068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3326..3914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4085..4945)
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /protein_id="AEI30146.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4085..4945)
                     /gene="bla"
                     /label=bla
     promoter        complement(4946..5050)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5396..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"