LOCUS       Exported                6668 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Functional Analysis vector pFA-HAH1-MAL2prom, complete sequence.
ACCESSION   HQ455520
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Single-Transformation Gene Function Test by Conditional Expression 
            of Both Alleles of Candida albicans Genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Paul S, Ganesan K.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2010) Institute of Microbial Technology, Sector 
            39-A, Chandigarh, UT 160036, India
REFERENCE   3  (bases 1 to 6668)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Functional Analysis vector pFA-HAH1-MAL2prom"
                     /mol_type="other DNA"
                     /db_xref="taxon:1096804"
     gene            47..915
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   562..915
     gene            921..2871
                     /gene="ARG4"
                     /label=ARG4
                     /note="derived from Candida albicans ARG4"
     CDS             1378..2784
                     /codon_start=1
                     /gene="ARG4"
                     /product="argininosuccinate lyase"
                     /EC_number="4.3.2.1"
                     /label=ARG4
                     /protein_id="AEQ75401.1"
                     /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADL
                     TGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGE
                     IIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMP
                     GYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREF
                     LAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLA
                     DAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLF
                     DALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISG
                     ECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGGTAKSAVLK
                     QLENLKSILS"
     gene            2876..3729
                     /gene="HIS1"
                     /label=HIS1
     repeat_region   2876..3229
     regulatory      3734..4269
                     /regulatory_class="promoter"
                     /note="MAL2 promoter from Candida albicans"
     promoter        complement(4306..4324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4582..5170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5341..6201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AEQ75402.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5341..6201)
                     /gene="bla"
                     /label=bla
     promoter        complement(6202..6306)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6652..2
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"