LOCUS       Exported                3465 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pF12K RM Flexi, complete sequence.
ACCESSION   EF030521
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Regulated Mammalian Expression System Technical Manual, TM289
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2006) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Mammalian expression vector pF12K RM Flexi"
                     /mol_type="other DNA"
                     /db_xref="taxon:410003"
     misc_feature    16..309
                     /label=12X lambda operator
                     /note="12X lambda operator"
     regulatory      322..434
                     /regulatory_class="promoter"
                     /note="minimal CMV promoter"
     CDS             477..812
                     /codon_start=1
                     /product="barnase"
                     /label=barnase
                     /note="lethal gene for selection"
                     /protein_id="ABK40093.1"
                     /translation="MAQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLAD
                     VAPGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHY
                     QTFTKIR"
     regulatory      973..1194
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     polyA_signal    982..1103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1502..2296
                     /codon_start=1
                     /product="aminoglycoside 3-phosphotransferase"
                     /function="kanamycin resistance"
                     /label=aminoglycoside 3-phosphotransferase
                     /protein_id="ABK40094.1"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      2465..2501
                     /label=ColE1-derived origin of replication
                     /note="ColE1-derived origin of replication"
     rep_origin      complement(2468..3056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      3172..3457
                     /regulatory_class="other"
                     /note="cer site; site for Escherichia coli XerCD 
                     recombinase"
     misc_feature    3172..3455
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"