LOCUS       Exported                7260 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  BAC cloning vector pEZ BAC, complete sequence.
ACCESSION   FJ160466
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Godiska R, Mead DA.
  TITLE     CopyRight pEZ BAC vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Godiska R, Mead DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2008) Lucigen Corp., 2120 W. Greenview Dr. #9, 
            Middleton, WI 53562, USA
REFERENCE   3  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="BAC cloning vector pEZ BAC"
                     /mol_type="other DNA"
                     /db_xref="taxon:561480"
     terminator      28..57
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_recomb     79..112
                     /label=loxP site #1
                     /note="loxP site #1"
     protein_bind    79..112
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    198..219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        234..264
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    272..288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     296..312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             308..580
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSYLGETIEYSSLHAIRIVNDGSDARANSPYSESYYNSLA
                     VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS"
     misc_feature    377..382
                     /label=HpaI blunt cloning site
                     /note="HpaI blunt cloning site"
     promoter        complement(411..429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(436..452)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     632..665
                     /label=loxP site #2
                     /note="loxP site #2"
     protein_bind    632..665
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     terminator      684..715
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     misc_feature    764..1162
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     misc_feature    958..1047
                     /label=lambda cosN site
                     /note="lambda cosN site"
     promoter        1185..1287
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1288..1947
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /label=cat
                     /note="chloramphenicol resistance"
                     /protein_id="ACI02340.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            1288..1947
                     /gene="cat"
                     /label=cat
     misc_feature    2218..2691
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(2764..3735)
                     /codon_start=1
                     /gene="parB"
                     /product="ParB"
                     /label=parB
                     /note="partitioning protein B; SopB"
                     /protein_id="ACI02341.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(2764..3735)
                     /gene="parB"
                     /label=parB
     CDS             complement(3735..4910)
                     /codon_start=1
                     /gene="parA"
                     /product="ParA"
                     /label=parA
                     /note="partitioning protein A; SopA"
                     /protein_id="ACI02342.1"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(3735..4910)
                     /gene="parA"
                     /label=parA
     misc_feature    5236..5486
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(5489..6244)
                     /codon_start=1
                     /gene="repE"
                     /product="RepE"
                     /label=repE
                     /note="replication protein"
                     /protein_id="ACI02343.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(5489..6244)
                     /gene="repE"
                     /label=repE
     rep_origin      6335..6554
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      6630..7244
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"