LOCUS       Exported                3643 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pExchange 2, complete sequence.
ACCESSION   AF327895
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Carstens C-P., Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2000) Technical Services, Stratagene, 11011 N 
            Torrey Pines Rd, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Expression vector pExchange 2"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="core vector designed to permit protein expression in
                     eukaryotic systems"
                     /db_xref="taxon:148366"
     regulatory      1..602
                     /regulatory_class="promoter"
                     /note="CMV immediate early promoter 1"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    694..721
                     /label=FLAG epitope tag
                     /note="FLAG epitope tag"
     CDS             699..722
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(746..762)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    759..794
                     /label=myc epitope tag
                     /note="myc epitope tag"
     CDS             762..791
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(821..839)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    852..1235
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1113..1234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1235..1696
                     /label=f1 origin
                     /note="f1 origin"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1723..1827
                     /gene="bla"
                     /label=AmpR promoter
     misc_binding    1837..1871
                     /label=CRE binding site
                     /bound_moiety="CRE"
                     /note="loxP site"
     protein_bind    complement(1842..1875)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(1917..2777)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AAK01701.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2947..3535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"