LOCUS       Exported                4188 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pEX302, complete sequence.
ACCESSION   FJ386489
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Zaltsman A, Krichevsky A, Loyter A, Citovsky V.
  TITLE     Agrobacterium induces expression of a host F-box protein required 
            for tumorigenicity
  JOURNAL   Cell Host Microbe 7 (3), 197-209 (2010)
  PUBMED    20227663
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Zaltsman A, Krichevsky A, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2008) Biochemistry and Cell Biology, SUNYSB, NY 
            11794, USA
REFERENCE   3  (bases 1 to 4188)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Cloning vector pEX302"
                     /mol_type="other DNA"
                     /db_xref="taxon:572047"
     primer_bind     71..87
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        94..112
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(127..246)
                     /codon_start=1
                     /product="VirE3 export signal"
                     /label=VirE3 export signal
                     /protein_id="ACJ12746.1"
                     /translation="MLNQLLSVPLPIPSPKPKSARSMIFEGSRPRERSTSRGF"
     primer_bind     249..265
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(299..315)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(332..870)
                     /regulatory_class="promoter"
                     /note="Agrobacterium virF promoter"
     promoter        complement(889..907)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(928..944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    952..968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(976..1006)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1021..1042
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(1111..2259)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2558..3352)
                     /codon_start=1
                     /gene="nptII"
                     /product="NptII"
                     /label=nptII
                     /note="KanR"
                     /protein_id="ACJ12745.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2558..3352)
                     /gene="nptII"
                     /label=nptII
     rep_origin      3561..4092
                     /label=oriV
                     /note="incP origin of replication"