LOCUS       Exported                6819 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Acinetobacter baumannii cloning vector pET-RA, complete sequence.
ACCESSION   HM219006
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Aranda J, Poza M, Gomez B, Rumbo S, Rumbo C, Parreira JR, Rodriguez 
            P, Bou G.
  TITLE     A rapid and simple method for constructing stable mutants of 
            Acinetobacter baumannii
  JOURNAL   BMC Microbiol. 10 (1), 279 (2010)
  PUBMED    21062436
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Poza M, Aranda J, Bou G.
  TITLE     pET-RA cloning vector for Acinetobacter baumannii
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6819)
  AUTHORS   Poza M, Aranda J, Bou G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2010) Microbiology, University Hospital A Coruna, 
            Spain, As Xubias, A Coruna, A Coruna 15006, Spain
REFERENCE   4  (bases 1 to 6819)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Acinetobacter baumannii cloning vector pET-RA"
                     /mol_type="other DNA"
                     /db_xref="taxon:765767"
     CDS             52..843
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=green fluorescent protein
                     /protein_id="ADH43299.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKESLKISQAVHAAHAEINEAGREVVG"
     CDS             52..765
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     rep_origin      860..2197
     terminator      2406..2492
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2584..2611
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2630..2721
                     /gene="bla"
                     /label=AmpR promoter
     gene            2730..3628
                     /gene="arr-2"
                     /label=arr-2
     promoter        2732..2836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3137..3628
                     /codon_start=1
                     /gene="arr-2"
                     /product="rifampin ADP-ribosylating transferase ARR-2"
                     /label=arr-2
                     /note="confers rifampin resistance"
                     /protein_id="ADH43300.1"
                     /translation="MLCSQIPTIKGLKMVKDWIPISHDNYKQVQGPFYHGTKANLAIGD
                     LLTTGFISHFEDGRILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDD
                     PNLTNKKFPGNPTQSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED
                     "
     rep_origin      3847..4435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4621..4761
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4863..5054)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"