LOCUS       Exported                8363 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pERV3, complete sequence.
ACCESSION   AF098284
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Cloning vector pERV3"
                     /lab_host="Escherichia coli K12"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:83610"
     rep_origin      complement(6..462)
                     /direction=LEFT
                     /label=f1(-) origin of replication
                     /note="f1(-) origin of replication"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     misc_feature    complement(463..901)
                     /label=SV40 polyA signal region
                     /note="SV40 polyA signal region"
     polyA_signal    479..600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          759..824
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(909..2348)
                     /codon_start=1
                     /product="retinoic acid receptor RXR"
                     /label=retinoic acid receptor RXR
                     /protein_id="AAC95154.1"
                     /translation="MKNTIDNMEQKLISEEDLDTKHFLPLDFSTQVNSSLTSPTGRGSM
                     AAPSLHPSLGPGIGSPGQLHSPISTLSSPINGMGPPFSVISSPMGPHSMSVPTTPTLGF
                     STGSPQLSSPMNPVSSSEDIKPPLGLNGVLKVPAHPSGNMASFTKHICAICGDRSSGKH
                     YGVYSCEGCKGFFKRTVRKDLTYTCRDNKDCLIDKRQRNRCQYCRYQKCLAMGMKREAV
                     QEERQRGKDRNENEVESTSSANEDMPVERILEAELAVEPKTETYVEANMGLNPSSPNDP
                     VTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDG
                     ILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKG
                     LSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKL
                     IGDTPIDTFLMEMLEAPHQMT"
     CDS             complement(2295..2324)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    complement(2334..2903)
                     /note="IRES; internal ribosome entry sequence"
     CDS             complement(2910..4760)
                     /codon_start=1
                     /product="ecdysone receptor VgEcR"
                     /label=ecdysone receptor VgEcR
                     /protein_id="AAC95155.1"
                     /translation="MLGERKDRASGYHYNALTCGSCKVFFRRSVTKSAVYCCKFGRACE
                     MDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGG
                     NGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIY
                     KLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAF
                     TKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIED
                     LLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHC
                     GDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQS
                     HLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSL
                     TQNDSQHQTQPQLQPQLPPQLQGQLQPQLHPQLQTQLQPQIQPQPQLLPVSAPVPASVT
                     APGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGG
                     NVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA"
     CDS             complement(4844..5077)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             complement(5078..5107)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      5107..5116
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      complement(5118..5744)
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     promoter        complement(5153..5356)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        5357..5660
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5745..6607)
                     /direction=LEFT
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"
     rep_origin      complement(5855..6443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6610..6940)
                     /label=thymidine kinase polyA signal region
                     /note="thymidine kinase polyA signal region"
     polyA_signal    6772..6819
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7051..7845)
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /function="neomycin resistance"
                     /label=neomycin phosphotransferase
                     /protein_id="AAC95153.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7880..8237)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7894..8029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(7945..8303)
                     /direction=LEFT
                     /label=SV40 origin of replication
                     /note="SV40 origin of replication"
     promoter        complement(8239..8343)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(8304..5)
                     /regulatory_class="promoter"
                     /note="Pbla promoter"