p10A1 expresses the 10A1 envelope protein under the control of the CMV immediate-early promoter (1).Virus packagedwith the 10A1 envelope protein can enter cells via two different surface molecules, the amphotropic retrovirus receptor or the GALV receptor, and therefore exhibits a broader host range than virus packaged with other envelope proteins (2, 3, 4). p10A1 includes IVS, a synthetic intron known to enhance the stability of the mRNA (5), the pUC origin of replication, and a bacterial ampicillin resistance (Ampr) gene for propagation and antibiotic selection in bacteria.
| 载体基本信息 | |||
| 载体名称 | p10A1 | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 6732 bp | 载体类型 | Retroviral Envelope Plasmids |
| 启动子 | CMV | ||
p10A1 载体载体图谱
p10A1 载体载体序列
LOCUS Exported 6732 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6732)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6732
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 97..476
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 477..680
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 792..1364
/note="beta-globin intron"
/note="intron from rabbit beta-globin gene"
polyA_site 3502..3557
/note="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(3915..3931)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3939..3955
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3963..3993)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4008..4029
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4317..4905)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5076..5936)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5937..6041)
/gene="bla"
/note="AmpR promoter"
rep_origin complement(6067..6522)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 6664..6680
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 6690..6708
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
