LOCUS       Exported                5370 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Genetically encoded fluorescent indicator for measuring NAD/NADH 
            ratio..
ACCESSION   .
VERSION     .
KEYWORDS    pC1-REX-YFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G, 
            Belousov VV
  TITLE     Genetically encoded fluorescent indicator for imaging NAD/NADH ratio
            changes in different cellular compartments.
  JOURNAL   Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 
            10.1016/j.bbagen.2013.11.018.
  PUBMED    24286672
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 
            10.1016/j.bbagen.2013.11.018."
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1067..1088
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1197..1218)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1458..1477)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2158..2279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2195..2214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2249..2268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2286..2741)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2423..2444)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2635..2654
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2768..2872
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2874..3231
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2897..2917)
                     /label=pBABE 3
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3082..3217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3144..3163
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3266..4060
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3320..3339)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3930..3949
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4248..4267)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4292..4339
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4668..5256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5157..5176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"