The pBC1 vector is a 21.6 kb vector designed to facilitate expression of recombinant proteins in the milk of transgenic animals. The pBC1 Milk Expression Vector Kit is intended for use in performing feasibility studies in mice, with the expectation that the user is interested in eventual large scale recombinant protein production using larger animals. Successful expression of recombinant protein in transgenic mice has generally been indicative of successful expression in larger animals such as goats or cows (Young et al., 1997). For feasibility studies, transgenic mice provide the added advantage of shorter generation times and faster evaluation than larger herd animals.
| 载体基本信息 | |||
| 载体名称 | pBC1 | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 21628 bp | 载体类型 | Milk Expression Vector |
| 克隆方法 | Enzyme digestion and ligation | ||
pBC1 载体载体图谱
pBC1 载体载体序列
LOCUS Exported 21628 bp ds-DNA circular SYN 13-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 21628)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..21628
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(15009..15020)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
promoter 15863..15967
/gene="bla"
/note="AmpR promoter"
CDS 15968..16828
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 16998..17586
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 17772..17912
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(18014..18205)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
terminator 20653..20747
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
