LOCUS       Exported                4048 bp ds-DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4048)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_RNA        94..112
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        133..151
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        203..221
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        242..260
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        312..330
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        351..369
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        421..439
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        460..478
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        530..548
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        569..587
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        639..657
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        678..696
                     /note="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     primer_bind     complement(954..970)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1183..1638
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1920..2024
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             2025..2885
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3056..3644
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3932..3953
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3968..3998
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4006..4022
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4030..4046
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"