LOCUS       Exported               21829 bp ds-DNA     circular SYN 13-JUL-2021
DEFINITION  Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap 
            genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo
            transduction of muscle, lung, liver and other tissues..
ACCESSION   .
VERSION     .
KEYWORDS    pDGM6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21829)
  AUTHORS   Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller
            DG, Russell DW, Chamberlain JS
  TITLE     Systemic delivery of genes to striated muscles using 
            adeno-associated viral vectors.
  JOURNAL   Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul 
            25.
  PUBMED    15273747
REFERENCE   2  (bases 1 to 21829)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nm1085"; 
            journalName: "Nat Med"; date: "2004-08"; volume: "10"; issue: "8"; 
            pages: "828-34"
FEATURES             Location/Qualifiers
     source          1..21829
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   21..123
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     211..361
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     primer_bind     483..499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     483..499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5268..5279
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      7501..7510
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     repeat_region   19726..19828
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(20019..20607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(20099..20118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(20759..21619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     21382..21401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(21620..21724)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     21792..21810
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"