LOCUS       Exported                6891 bp ds-DNA     circular SYN 29-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBABEpuro U3-hTR-500
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6891)
  AUTHORS   Wong JM, Collins K
  TITLE     Telomerase RNA level limits telomere maintenance in X-linked 
            dyskeratosis congenita.
  JOURNAL   Genes Dev. 2006 Oct 15. 20(20):2848-58.
  PUBMED    17015423
REFERENCE   2  (bases 1 to 6891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 Oct 15. 20(20):2848-58."
FEATURES             Location/Qualifiers
     source          1..6891
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..470
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    535..734
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             935..1351
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1281..1303
                     /label=pLXSN 5
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1319..1335
                     /label=pBABE 5
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1409..1429)
                     /label=pBABE 3
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1414..1743
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1594..1729
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1656..1675
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1753..2352
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1753..1772)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2249..2269
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3825..3841)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3825..3841)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3873..3892)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3874..3892)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3899..3916)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3899..3915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3908..3930)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4144..4163)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     LTR             4378..4847
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4977..4994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5148..5736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5228..5247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5896..6756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6519..6538
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6757..6861)
                     /gene="bla"
                     /label=AmpR promoter