LOCUS       Exported                4396 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  luciferase reporter for TERT promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.0-TERT WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Bell RJ, Rube HT, Kreig A, Mancini A, Fouse SD, Nagarajan RP, Choi 
            S, Hong C, He D, Pekmezci M, Wiencke JK, Wrensch MR, Chang SM, Walsh
            KM, Myong S, Song JS, Costello JF
  TITLE     Cancer. The transcription factor GABP selectively binds and 
            activates the mutant TERT promoter in cancer.
  JOURNAL   Science. 2015 May 29;348(6238):1036-9. doi: 10.1126/science.aab0015.
            Epub 2015 May 14.
  PUBMED    25977370
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aab0015"; journalName: "Science"; date: 
            "2015-05-29- 29"; volume: "348"; issue: "6238"; pages: "1036-9"
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    33..81
                     /note="synthetic polyadenylation signal"
     CDS             186..1046
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1246..1834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1735..1754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1988..2005
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2253..2374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2290..2309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2344..2363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(2418..4070)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      4067..4076
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    join(4324..4396,1..19)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(4356..4375)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"