LOCUS       Exported                5836 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEnEOiLUCThsp, complete sequence.
ACCESSION   KF545094
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Fukudome A, Aksoy E, Wu X, Kumar K, Jeong IS, May K, Russell WK, 
            Koiwa H.
  TITLE     Arabidopsis CPL4 is an essential C-terminal domain phosphatase that 
            suppresses xenobiotic stress responses
  JOURNAL   Plant J. 80 (1), 27-39 (2014)
  PUBMED    25041272
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Koiwa H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2013) Department of Horticultural Science, Texas 
            A&M University, MS2133 TAMU, College Station, TX 77843-2133, USA
REFERENCE   3  (bases 1 to 5836)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Cloning vector pEnEOiLUCThsp"
                     /mol_type="other DNA"
                     /db_xref="taxon:1427727"
     rep_origin      complement(63..651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(741..1550)
                     /codon_start=1
                     /product="KanR"
                     /label=KanR
                     /protein_id="AHA91193.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     protein_bind    1673..1772
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     1674..1769
                     /label=AttL2
                     /note="AttL2"
     regulatory      1791..2455
                     /regulatory_class="promoter"
                     /note="Arabidopsis UBQ1 promoter"
     regulatory      1798..2551
                     /regulatory_class="promoter"
                     /note="enhanced cauliflower mosaic virus 35S promoter EL2"
     misc_feature    2555..2625
                     /label=Tobacco mosaic virus omega
                     /note="Tobacco mosaic virus omega"
     intron          2642..3236
                     /note="EF1a-intron"
     CDS             3172..4824
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=firefly luciferase
                     /protein_id="AHA91194.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     misc_recomb     4262..4357
                     /label=AttL1
                     /note="AttL1"
     3UTR           4833..4992
                     /label=HSP18.2 3UTR
                     /note="HSP18.2 3UTR"
     regulatory      4993..5082
                     /regulatory_class="terminator"
                     /note="HSP18.2 terminator"
     regulatory      5097..5361
                     /regulatory_class="terminator"
                     /note="Nos terminator"
     terminator      5107..5359
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(5380..5479)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      5529..5556
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      5648..5734
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"