LOCUS       Exported                5177 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEnEL2Omega-LUC, complete sequence.
ACCESSION   JN570503
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Aksoy E, Jeong IS, Koiwa H.
  TITLE     Loss of function of Arabidopsis C-terminal domain phosphatase-like1 
            activates iron deficiency responses at the transcriptional level
  JOURNAL   Plant Physiol. 161 (1), 330-345 (2013)
  PUBMED    23144187
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Koiwa H.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2011) Dept. Horticultural Sciences, Texas A&M 
            University, MS2133 Texas A&M University, College Station, TX 
            77843-2133, USA
REFERENCE   3  (bases 1 to 5177)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Cloning vector pEnEL2Omega-LUC"
                     /mol_type="other DNA"
                     /db_xref="taxon:1129353"
     rep_origin      complement(63..651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(741..1550)
                     /codon_start=1
                     /product="KanR"
                     /label=KanR
                     /note="kanamycin resistance"
                     /protein_id="AEX00084.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     protein_bind    1673..1772
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     1674..1769
                     /label=attL2
                     /note="attL2"
     regulatory      1798..2551
                     /regulatory_class="promoter"
                     /note="enhanced cauliflower mosaic virus 35S promoter EL2"
     promoter        2506..2551
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    2555..2630
                     /label=Tobacco Mosaic Virus omega sequence
                     /note="Tobacco Mosaic Virus omega sequence"
     CDS             2637..4289
                     /codon_start=1
                     /gene="LUC"
                     /product="firefly luciferase"
                     /label=LUC
                     /protein_id="AEX00085.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     gene            2637..4289
                     /gene="LUC"
                     /label=LUC
     regulatory      4432..4709
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      4448..4700
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(4721..4820)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_recomb     complement(4724..4819)
                     /label=attL1
                     /note="attL1"
     terminator      4870..4897
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      4989..5075
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"